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MABF572

Sigma-Aldrich

Anti-CD4 Antibody (human), APC, clone OKT4

clone OKT4, from mouse

Synonym(s):

T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD antigen CD4

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

Allophycocyanin conjugate

antibody form

purified antibody

antibody product type

primary antibodies

clone

OKT4, monoclonal

species reactivity

human

packaging

pkg of 5 μL (0.125 μg/test)

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2bκ

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CD4(920)

General description

The OKT4 antibody reacts with human CD4, a 59 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4, and co-receptors CCR5 and CXCR4, may also be utilized by HIV-1 to enter T cells. Human CD4 is typically expressed on thymocytes, some mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells. The OKT4 antibody is widely used as a phenotypic marker for CD4 expression. It is cross-reactive with CD4 in several non-human species, including Chimpanzee, Cynomolgus and Rhesus. This antibody recognizes a different epitope, and thus does not block binding of, the alternative Anti-Human CD4 antibody clone RPA-T4 (Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061-4065).

Specificity

This antibody recognizes a different epitope, and thus does not block binding of Anti-human CD4, clone RPA-T4.

Application

This mouse monoclonal antibody Anti-CD4 (human), APC & clone OKT4 validated for use in western blotting, IP, ICC, Immunofluorescence and Flow Cytometry for the detection of CD4.

Quality

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.125 µg of this antibody detected CD4 in one million human peripheral blood lymphocytes.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Cristiano Scottà et al.
Haematologica, 101(1), 91-100 (2015-10-17)
Immunosuppressive drugs in clinical transplantation are necessary to inhibit the immune response to donor antigens. Although they are effective in controlling acute rejection, they do not prevent long-term transplant loss from chronic rejection. In addition, immunosuppressive drugs have adverse side

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