biological source
rat
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
1H2, monoclonal
mol wt
calculated mol wt 56.89 kDa
observed mol wt ~57 kDa
species reactivity
human
packaging
antibody small pack of 100 μL
technique(s)
immunofluorescence: suitable
western blot: suitable
isotype
IgG2aκ
UniProt accession no.
shipped in
dry ice
storage temp.
2-8°C
target post-translational modification
unmodified
General description
Receptor-interacting serine/threonine-protein kinase 3 (UniProt: Q9Y572; also known as EC:2.7.11.1, RIP-like protein kinase 3, Receptor-interacting protein 3, RIP-3) is encoded by the RIPK3 (also known as RIP3) gene (Gene ID: 11035) in human. RIPK3 is a serine/threonine protein kinase that is expressed in embryo and adult spleen, liver, testis, brain, and lung. Its protein kinase domain is localized in amino acids 21 to 287 in the N-terminal half and it also has a C-terminal RIP homolytic interaction motif (RHIM; aa 450-466), which mediates interaction with the RHIM motif of RIPK1 and facilitate the formation of a necrosome. RIPK3 is shown to activate both necroptosis and apoptosis. Activated RIPK3 forms a necrosis-inducing complex and mediates phosphorylation of MLKL, promoting MLKL localization to the plasma membrane and execution of programmed necrosis that is characterized by calcium influx and plasma membrane damage. Its location is mainly cytoplasmic, however, following infection, it can also be detected in the nucleus. RIPK3 also regulates apoptosis that is dependent on RIPK1, FADD, and CASP8, and is independent of MLKL and RIPK3 kinase activity. It phosphorylates RIPK1 and then RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation. In some cell types, RIPK3 is also reported to restrict viral replication by promoting cell death-independent responses. Clone 8G7 is raised against an extended kinase domain (residues 2-353) of mouse RIPK3 and can detect forms that may not be detected by antibodies directed towards the C-terminus of the full-length RIPK3. (Ref.: Samson, AL., et al. (2021). Cell Death Differ. https://doi.org/10.1038/s41418-021-00742-x; Petrie, EJ., et al. (2019). Cell Rep. 28(13); 3309-3319).
Specificity
Clone 1H2 is a rat monoclonal antibody that detects human Receptor-interacting serine/threonine-protein kinase (RIP3). It targets an epitope within the extended kinase domain that includes most of the N-terminal half and a part of C-terminal half.
Immunogen
Recombinant fragment corresponding to 355 amino acids from the extended kinase domain of human Receptor-interacting serine/threonine-protein kinase 3 (RIPK3).
Application
Quality Control Testing
Evaluated by Western Blotting in HT-29 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected RIPK3/RIP3 in HT-29 cell lysate.
Tested Applications
Western Blotting Analysis: A representative lot detected RIPK3/RIP3 in Western Blotting applications (Samson, A.L., et. al. (2020). Nat Commun. 11(1):3151).
Immunofluorescence Analysis: A representative lot detected RIPK3/RIP3 in Immunofluorescence applications (Samson, A.L., et. al. (2021). Cell Death Differ. https://doi.org/10.1038/s41418-021-00742-x).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in HT-29 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected RIPK3/RIP3 in HT-29 cell lysate.
Tested Applications
Western Blotting Analysis: A representative lot detected RIPK3/RIP3 in Western Blotting applications (Samson, A.L., et. al. (2020). Nat Commun. 11(1):3151).
Immunofluorescence Analysis: A representative lot detected RIPK3/RIP3 in Immunofluorescence applications (Samson, A.L., et. al. (2021). Cell Death Differ. https://doi.org/10.1038/s41418-021-00742-x).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-RIPK3/RIP3, clone 1H2, Cat. No. MABC1640, is a rat monoclonal antibody that detects RIPK3/RIP3 and is tested for use in Immunofluorescence and Western Blotting.
Physical form
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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