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Key Documents

AB5908

Sigma-Aldrich

Anti-Potassium Channel HERG Antibody, CT

Chemicon®, from rabbit

Synonym(s):

Erg1, Kcnh2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... KCNH2(3757)

Specificity

Recognizes HERG (hKv11.1, Voltage gated K+ channel subfamily H member 2, KCNH2, ether-a-go-go related channel). The antibody recognizes the HERG1b splice variant but not splice variants HERG1-3 or HERG-4. The immunogen shares similiarity with rat erg2 (22/54) and rat erg3 (21/54).

Immunogen

Epitope: C-terminus
GST fusion protein corresponding to amino acids 1106-1159 of human erg1 (HERG) (Accession number Q12809).

Application

Anti-Potassium Channel HERG Antibody, C-terminus is an antibody against Potassium Channel HERG for use in IC, IP & WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Western blot: 1:200-400 using ECL on HERG transfected HEK293 cells.

Immunocytochemistry

Immunoprecipitation

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Target description

127 kDa

Physical form

Affinity Purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
ImmunoAffinity Purified

Storage and Stability

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 120 μg of fusion protein (35 kD). The stock solution of the antigen can be made up using 100 μL of PBS. For negative control, preincubate 3 μg of fusion protein with 1 μg of antibody for one hour at room temperature. For positive control, use 20 ng of fusion protein per lane. Optimal concentrations must be determined by the end user.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Hazard Statements

Precautionary Statements

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Samir Saba et al.
Heart rhythm, 5(1), 124-130 (2008-01-09)
Biventricular (BIV) pacing can improve cardiac function in heart failure (HF). This study sought to investigate the mechanisms of benefit of BIV pacing using a rabbit model of myocardial infarction (MI). New Zealand White rabbits were divided into 4 groups
Jian-Hua Gong et al.
Oncology reports, 23(6), 1747-1756 (2010-04-30)
Potassium channels are essential for the regulation of cell proliferation. As reported, HERG protein is overexpressed in a wide range of human tumors, including colon carcinoma. The aim of this study was to investigate the effects of antibacterial agents sparfloxacin
Jochen Rose et al.
American journal of physiology. Heart and circulatory physiology, 288(5), H2077-H2087 (2005-01-08)
Action potential (AP) prolongation is a hallmark of failing myocardium. Functional downregulation of K currents is a prominent feature of cells isolated from failing ventricles. The detailed changes in K current expression differ depending on the species, the region of
Fadi G Akar et al.
American journal of physiology. Heart and circulatory physiology, 288(6), H2887-H2896 (2005-02-01)
Heart failure (HF) is characterized by marked prolongation of action potential duration and reduction in cellular repolarization reserve. These changes are caused in large part by HF-induced K(+) current downregulation. Molecular mechanisms underlying these changes remain unclear. We determined whether
Karen M Hurley et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 26(40), 10253-10269 (2006-10-06)
Type I vestibular hair cells have large K+ currents that, like neuronal M currents, activate negative to resting potential and are modulatable. In rodents, these currents are acquired postnatally. In perforated-patch recordings from rat utricular hair cells, immature hair cells

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