AB5458
Anti-Neprilysin Antibody (Neutral Endopeptidase, Nep)
serum, Chemicon®
Synonym(s):
neprilysin, Neprilysin, Enkephalinase, enkephalinase, atriopeptidase, Atriopeptidase, Neutral Endopeptidase, NEP, Endopeptidase 24.11, CD10
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, mouse, human
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... MME(4311)
General description
Human neutral endopeptidase (Neprilysin) is single pass type II integral membrane endopeptidase that functions to activate and degrade biologically active enzymes. Endopeptidase,in general, function to cleave peptide bonds within enzyme sequences, in contrast to exopeptidases that target the end sequence. Neprilysin is part of the peptidase M13 family that acts on polypeptides of less than 30 amino acids with Thermolysin-like specificity for cleave sites. Thermolysin is thought to cleave before amino acids Ile, Met, Phe, Trpm or Val (unless Pro precedes) and after Ala, Asp, His, or Thr. Neprilysin preferentially cleaves polypeptides between hydrophobic residues, particularly with Phe or Tyr at position 1. Neprilysin research has found it important in the degradation of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Research has found it may function in the degradation of atrial natriuretic factor (ANF). Upregulation of ANF functions to regulate homeostatis of water, sodium, potassium and adipose in response to high blood pressure in muscle cells of the heart.
Specificity
Reactivity with other species has not been confirmed.
Recognizes Neprilysin (Neutral Endopeptidase, NEP). On western blots of rat kidney extracts the antibody detects a band migrating at approximately 86 kDa corresponding to neprilysin.
Immunogen
Rat Neprilysin.
Application
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
This Anti-Neprilysin Antibody (Neutral Endopeptidase, Nep) is validated for use in IH, WB for the detection of Neprilysin (Neutral Endopeptidase).
Western Blotting:
A previous lot of this antibody was used on Western Blotting.
Immunohistochemistry:
A previous lot of this antibody was used on Immunohistochemistry.
Optimal working dilutions must be determined by the end user.
A previous lot of this antibody was used on Western Blotting.
Immunohistochemistry:
A previous lot of this antibody was used on Immunohistochemistry.
Optimal working dilutions must be determined by the end user.
Target description
~86 kDa
Physical form
Rabbit polyclonal serum. Contains no preservative.
Unpurified
Storage and Stability
Stable for 1 year at -20ºC from date of receipt.
Analysis Note
Control
Kidney tissue.
Kidney tissue.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Hyun-Ju Lee et al.
Biology, 10(9) (2021-09-29)
The coenzyme Q10 analogue idebenone is an FDA-approved antioxidant that can cross the blood-brain barrier (BBB). The effects of idebenone on the pathology of Alzheimer's disease (AD) and the underlying molecular mechanisms have not been comprehensively investigated. Here, we examined
Yasir AlSiraj et al.
The Journal of pharmacology and experimental therapeutics, 377(3), 326-335 (2021-03-13)
Combined neprilysin (NEP) inhibition (sacubitril) and angiotensin type 1 receptor (AT1R) antagonism (valsartan) is used in the treatment of congestive heart failure and is gaining interest for other angiotensin II (AngII)-related cardiovascular diseases. In addition to heart failure, AngII promotes
H Benson Peng et al.
Drug metabolism and disposition: the biological fate of chemicals, 48(10), 944-955 (2020-08-08)
Amyloid-β peptides of 40 and 42 amino acid lengths, which are synthesized in neurons and degraded in the brain and liver, have the potential to aggregate and form neuritic plaques in Alzheimer disease. The kinetics of human amyloid-β (hAβ) 40
A Casselbrant et al.
Journal of the renin-angiotensin-aldosterone system : JRAAS, 16(3), 578-586 (2014-01-29)
Angiotensin II (AngII) elicits smooth muscle contractions via activation of AngII type 1 receptor (AT1R) in the intestinal wall and in sphincter regions in several species. Achalasia is a rare swallowing disorder and is characterized by a loss of the
Carlos P Vio et al.
Frontiers in physiology, 9, 1791-1791 (2019-01-09)
Chronic hypoxia has been postulated as one of the mechanisms involved in salt-sensitive hypertension and chronic kidney disease (CKD). Kidneys have a critical role in the regulation of arterial blood pressure through vasoactive systems, such as the renin-angiotensin and the
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