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Key Documents

18-160

Millipore

Protein A, HRP conjugate

1 mg Protein A, HRP conjugate for Dot Blot, ELISA, Western Blotting & IHC.

Synonym(s):

detection reagent

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About This Item

UNSPSC Code:
41105332
eCl@ss:
32160405
NACRES:
NB.22
Pricing and availability is not currently available.

form

solid

manufacturer/tradename

Upstate®

technique(s)

ELISA: suitable
dot blot: suitable
immunohistochemistry: suitable
western blot: suitable

shipped in

wet ice

Related Categories

Application

Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy, and immunohistochemistry.

Physical form

chromatographically pure Protein A conjugated to horseradish peroxidase lyophilized from 0.02 M Potassium Phosphate, 0.15M NaCl, pH 7.2, 10 mg/ml BSA- Immunoglobulin and Protease free

Storage and Stability

Lyophilized: Stable for 2 years at -20°C from date of shipment. Rehydrated: Stable for 6 months at -20°C.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Fabrizio Piazza et al.
Annals of neurology, 73(4), 449-458 (2013-04-30)
Cerebral amyloid angiopathy-related inflammation (CAA-ri) is characterized by vasogenic edema and multiple cortical/subcortical microbleeds, sharing several aspects with the recently defined amyloid-related imaging abnormalities (ARIA) reported in Alzheimer's disease (AD) passive immunization therapies. Herein, we investigated the role of anti-amyloid
Om Prakash et al.
Indian journal of microbiology, 57(2), 218-225 (2017-06-15)
An endophytic species of Micrococcus was isolated from Aloe vera leaf (syn. Aloe barbadensis) and screened for protease production with five other species of Micrococcus. Data indicated that endophytic Micrococcus aloeverae AE-6 MCC 2184T and Micrococcus yunnanensis DSM 21948T showed
Xiaohong Liu et al.
The Journal of biological chemistry, 291(16), 8440-8452 (2016-01-31)
T cell activation following antigen binding to the T cell receptor (TCR) involves the mobilization of intracellular Ca(2+) to activate the key transcription factors nuclear factor of activated T lymphocytes (NFAT) and NF-κB. The mechanism of NFAT activation by Ca(2+)
Maria Caterina Rotiroti et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 28(9), 1974-1986 (2020-06-12)
The successful implementation of chimeric antigen receptor (CAR)-T cell therapy in the clinical context of B cell malignancies has paved the way for further development in the more critical setting of acute myeloid leukemia (AML). Among the potentially targetable AML
Anita Hryniewicz-Jankowska et al.
International journal of molecular sciences, 22(4) (2021-02-13)
Inhibition of the protein neddylation process by the small-molecule inhibitor MLN4924 has been recently indicated as a promising direction for cancer treatment. However, the knowledge of all biological consequences of MLN4924 for cancer cells is still incomplete. Here, we report
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Questions

1–2 of 2 Questions  

  1. What concentration of use do you suggest for ELISA?

    1 answer

    1. A working dilution of 1:20,000 to 1:100,000 is suggested for ELISA.
      Please see the link below to review the available Certificates of Analysis:
      https://www.emdmillipore.com/US/en/product/Protein-A-HRP-conjugate,MM_NF-18-160#anchor_COA

      Helpful?

  2. How to rehydrate? Please give me some suggestions about Protein A (Product No.18-160). Thank you!

    1 answer

    1. To rehydrate, add 500 µL sterile, distilled water to make a 2 mg/mL stock solution. Solutions are stable for 6 months at -20°C. Aliquot to avoid repeated freeze/thaw cycles.

      Helpful?

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