809705

5-DBCO-PEG₄-dUTP, 10mM Aqueous Solution

• Synonym(s): 5-DBCO-PEG₄-dUTP
• Empirical Formula (Hill Notation): C₄₂H₅₄N₅O₂₁P₃
• Molecular Weight: 1057.82
• UNSPSC Code: 12352200
• PubChem Substance ID: 329769180
• NACRES: NA.22

Properties

• Assay: >95% (HPLC)
• Quality Level: 200
• form: solution
• reaction suitability: reaction type: click chemistry
• solubility: Tris buffer: 100 mM (pH 7.5)
• shipped in: wet ice
• storage temp.: −20°C
• SMILES string: O=C1NC(N([C@H]2C[C@@H]([C@@H](COP(O)(OP(O)(OP(O)(O)=O)=O)=O)O2)O)C=C1/C=C\CNC(CCOCCOCCOCCOCCNC(CCC(N3CC4=CC=CC=C4C#CC5=CC=CC=C53)=O)=O)=O)=O
• InChI: 1S/C42H54N5O21P3/c48-35-26-40(66-36(35)29-65-70(57,58)68-71(59,60)67-69(54,55)56)47-28-33(41(52)45-42(47)53)9-5-16-43-38(50)15-18-61-20-22-63-24-25-64-23-21-62-19-17-44-37(49)13-14-39(51)46-27-32-8-2-1-6-30(32)11-12-31-7-3-4-10-34(31)46/h1-10,28,35-36,40,
• InChI key: DINRDGNWAWZURW-AWZRJCEASA-N

Description

Application

5-DBCO-PEG4-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR with Taq polymerase. It is enzymatically incorporated into DNA/cDNA as a substitute for its natural counterpart dTTP. The resulting DBCO-functionalized DNA/cDNA can be labeled with azide-labeled molecules using copper-free click chemistry. This enables experiments such as introduction of a biotin group for purification tasks, introduction of a fluorescent group for detection, or crosslinking the DNA to azide-functionalized biomolecules such as proteins.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2