SAB4200082

Monoclonal Anti-Maltose Binding Protein (MBP) antibody produced in rat

1.0 mg/mL, clone MBP 7G4, purified immunoglobulin

• Synonym(s): Anti-MBP
• MDL number: MFCD00282353
• UNSPSC Code: 12352203
• NACRES: NA.56

Properties

• biological source: rat
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: MBP 7G4, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~42 kDa
• species reactivity: wide range
• concentration: 1.0 mg/mL
• technique(s): immunoprecipitation (IP): suitable; indirect ELISA: suitable; western blot: 0.1-0.2 μg/mL using MBP recombinant protein
• isotype: IgG2a
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

MBP is part of a large class of proteins that aid in the uptake of small molecules. While it naturally resides in the periplasm, MBP can also be expressed at high yields in the cytoplasm. For different proteins, increased solubility, enhanced stability and markedly improved yields have been reported after fusion to MBP.

Monoclonal Anti-Maltose Binding Protein (MBP) (rat IgG2a isotype) is derived from the hybridoma MBP 7G4 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with MBP-fusion protein.1 The antibody is purified from culture supernatant of hybridoma cells grown in a bioreactor. Monoclonal Anti- Maltose Binding Protein (MBP) is specific for MBP.

Specificity

Monoclonal Anti- Maltose Binding Protein (MBP) is specific for MBP.

Immunogen

MBP-fusion protein

Application

Monoclonal Anti-Maltose Binding Protein (MBP) antibody produced in rat is suitable for:

• immunoblotting
• enzyme linked immunosorbent assay (ELISA)
• immunoprecipitation

Monoclonal antibodies recognizing specifically MBP are useful in various immunotechniques for identifying the expression of an MBP fusion protein in bacteria or in cells transfected with MBP fusion protein expressing vectors.

Biochem/physiol Actions

Maltose binding protein (MBP) tag is known to be used in genetic engineering to create a stable fusion product that does not appear to interfere with the bioactivity of the protein of interest or with the biodistribution of the MBP tagged product. The expression of polypeptides in-frame with maltose binding protein (MBP) allows for their easy purification from bacterial extracts under mild conditions, which employ a single affinity chromatographic step on amylose resin. This system and others based on the expression of fusion proteins utilize a specific protease cleaving site to facilitate correct cleavage of the fusion protein. Thus, the MBP system incorporates a factor Xa cleavage site at the carboxy terminus of the MBP sequence, and cleavage by factor Xa separates MBP from its partner protein. Many recombinant proteins have been engineered with MBP tags to facilitate the detection, isolation and purification of the proteins.

Physical form

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at -20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended use, freeze at -20 °Cin working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable