This product has not been tested in-house for the application of assessing the effect of enzymes in pancreatin on fungus and fungal cell walls, specifically with lipase. A recommended concentration has not been established. Conducting a literature search may provide guidance on this matter.
P7545
Pancreatin from porcine pancreas
8 × USP specifications
Synonym(s):
Pancreatin from hog pancreas
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About This Item
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form
powder
Quality Level
specific activity
8 × USP specifications
contains
lactose as extender
storage temp.
−20°C
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Application
Pancreatin is a mixture of several digestive enzymes produced by the exocrine cells of the porcine pancreas. It is a broad-spectrum protease composed of amylase, trypsin, lipase, ribonuclease and protease. It is used for in vitro digestibility analysis[1]. Product P7545 has been used to test the sensitivities of cellulolytic bacteria inhibitors[2].
The enzyme has been used along with amyloglucosidase for the in vitro digestion of starch in food samples.[3] Pancreatin from Sigma has been used for in vitro pepsin-pancreatin digestion assays while estimating of lower gut digestibility using rumen feed residues.[4] It has also been used along with pepsin to simulate in vitro gastric and ileal digestion of raw materials in pigs.[5]
Biochem/physiol Actions
Due to the enzymatic components such as trypsin, amylase and lipase, pancreatin hydrolyzes proteins, starch and fats. Pancreatin will convert not less than 25 times its weight of potato starch into soluble carbohydrates in 5 minutes in water at 40 °C, will digest not less than 25 times its weight of casein in 60 minutes at pH7.5 at 40 °C and will release not less than 2 microequivalents of acid per min per mg pancreatin from olive oil at pH9.0 at 37 °C.
Pancreatin contains enzymatic components including trypsin, amylase and lipase, ribonuclease, and protease, produced by the exocrine cells of the porcine pancreas. This combination of enzymes allows it to hydrolyze proteins, starch and fats. Pancreatin will convert not less than 25 times its weight of potato starch into soluble carbohydrates in 5 minutes in water at 40°C, will digest not less than 25 times its weight of casein in 60 minutes at pH 7.5 at 40°C and will release not less than microequivalents of acid per min per mg pancreatin from olive oil at pH 9.0 at 37°C.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
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Hello, I want to assess the effect of enzymes in pancreatin on fungus and fungal cell wall specially (with lipase), is there a recommended concentration to use for pancreatin solution? and what is the recommended dissolving solution in this case? Thanks
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Excuse me, is it possible to provide protease activity report for buyer? thank you very much and look forward to your reply.
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This product meets 8X the minimum USP specifications. According to the USP monograph for Pancreatin, the 1X minimum specification is 25 USP units amylase, 2 USP units lipase, and 25 USP units protease per milligram. The calculated protease activity for the 8X preparation then, would be at least 200 units per milligram. This is a calculated value based on the USP monograph and are not reported separately on the Certificate of Analysis.
Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/p7545#product-documentationHelpful?
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Hi, what is the dissovled soultion? It can't dissolved in water or 1 mM HCl
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This product is not tested for solubility. For assay purposes. it is dissolved at 40 mg/3mL PBS, pH 6.8. Trituration is required for 5 - 10 minutes. Historically, this product has also been dissolved in water at 20 mg/ml. with heating at 37 °C. This method yields a hazy solution.
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Is the product used for digestion cells in clinical laboratory? Can you please help me?
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This product is for research use only and is not suitable for in vitro diagnostic studies.
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What is the solubility of this enzyme in water?
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This product is soluble in water at up to 20 mg/ml in water. Heat may be required. This concentration will yield a hazy solution.
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What is the shelf life for this product?
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This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one-year warranty from the date of shipment. For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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Bonjour, Que signifie exactement : '8 x SP specifications' ? Merci pour votre réponse
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The specific activity of this pancreatin is specified to be at least 8 x the minimum USP standard.
The USP defines a minimum specification of a 1 X Pancreatin as follows:
- 25 USP protease units per mg.
- 25 USP amylase units per mg.
- 2 USP lipase units per mg.Therefore, activity will be 8 times that.
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