L5385
α-Lactalbumin from bovine milk
Type I, ≥85% (PAGE), lyophilized powder
Synonym(s):
Bos d 4, Lactose synthase B protein, alpha-lactalbumin
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About This Item
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biological source
bovine milk
Quality Level
type
Type I
Assay
≥85% (PAGE)
form
lyophilized powder
technique(s)
cell culture | mammalian: suitable
electrophoresis: suitable
solubility
H2O: soluble 10 mg/mL, clear to slightly hazy, colorless to faintly yellow
UniProt accession no.
storage temp.
−20°C
Gene Information
cow ... LALBA(281894)
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General description
α-Lactalbumin is a small, globular, whey protein that has been found in all milk studied to date. It is a metalloprotein of approximately 14 kDa produced in the mammary glands.
Application
α-Lactalbumin from bovine milk has been used as a supplement of basal medium for various cell cultures. It has also been used as a marker for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
Biochem/physiol Actions
α-Lactalbumin is the cheif protein in human milk. It consists of a single polypeptide chain with 8 cysteines which form disulfide bridges. α-Lactalbumin binds several metal ions, including calcium, which is thought to play a role in the regeneration of native α-lactalbumin from the reduced, denatured form. α-Lactalbumin also has a distinct zinc binding site that is thought to play a role in the binding of the lactose synthase complex. The mature protein consists of 123 amino acid residues (14 kD), and it has a three-dimensional structure with 1.7 Α° resolution, demonstrating four α-helices and a triple stranded antiparallel β-sheet.
Alters the substrate specificity of galactosyltransferase to increase the rate of lactose formation; the complex of galactosyltransferase and α-lactalbumin is called lactose synthase. Site-directed mutagenesis of Asp87 or Asp88 to Ala completely abolishes the strong calcium binding affinity and reduces the stimulation of lactose synthase to <3.5% of the maximal rate.
Quality
Calcium saturated. May have traces of ammonium sulfate and sodium phosphate
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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The Journal of biological chemistry, 256(16), 8582-8587 (1981-08-25)
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