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A4058

Sigma-Aldrich

Acrylamide solution

40%, suitable for electrophoresis, sterile-filtered

Synonym(s):

2-Propenamide, 40% Acrylamido solution, Acrylic amide, Akrylamid, Polyacrylamide solution

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About This Item

Linear Formula:
CH2=CHCONH2
CAS Number:
79-06-1
Molecular Weight:
71.08
Beilstein:
6185892
MDL number:
MFCD00008032
UNSPSC Code:
41105319
eCl@ss:
39031205
PubChem Substance ID:
24890742
NACRES:
NB.22
Pricing and availability is not currently available.

sterility

sterile-filtered

Quality Level

200

form

liquid

concentration

40%

technique(s)

electrophoresis: suitable

storage temp.

2-8°C

SMILES string

NC(=O)C=C

InChI

1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)

InChI key

HRPVXLWXLXDGHG-UHFFFAOYSA-N

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General description

Acrylamide has been classified as probably carcinogenic in humans. It is formed in fried and oven-cooked foods due to Maillard reaction between amino acids and reducing sugars.[1]

Application

Acrylamide solution is suitable for use in electrophoresis. It may be used in the preparation of gel buffer for the determination of molecular weight of polypeptide chains by polyacrylamide gel electrophoresis.[2] It may be used in the electrochemical polymerization of pyrrole.[3] It may be used to prepare the pH 7.6 gels, required for the determination of molecular weights of histone by sodium dodecyl sulfate gel electrophoresis.[4]

Preparation Note

Prepared from electrophoresis grade acrylamide in ultra-pure water.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

Target Organs

Peripheral nervous system

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000313015
0000295139
0000330001
0000305330
0000330001

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The molecular weights of vertebrate histones exploiting a modified sodium dodecyl sulfate electrophoretic method.
S Panyim et al.
The Journal of biological chemistry, 246(24), 7557-7560 (1971-12-25)
Donald S Mottram et al.
Nature, 419(6906), 448-449 (2002-10-09)
Reports of the presence of acrylamide in a range of fried and oven-cooked foods have caused worldwide concern because this compound has been classified as probably carcinogenic in humans. Here we show how acrylamide can be generated from food components
Electrochemical polymerization of pyrrole in acrylamide solution.
Sarac AS, et al.
Synthetic Metals, 98(3), 177-182 (1999)
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
K Weber et al.
The Journal of biological chemistry, 244(16), 4406-4412 (1969-08-25)
Jonas S Laursen et al.
Journal of the American Chemical Society, 135(7), 2835-2844 (2013-01-25)
Non-natural peptide analogs have significant potential for the development of new materials and pharmacologically active ligands. One such architecture, the β-peptoids (N-alkyl-β-alanines), has found use in a variety of biologically active compounds but has been sparsely studied with respect to
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Questions

1–4 of 4 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. What is the ratio of acrylamide:bisacrylamide in this product? Is it 37.5:1, 29:1 or 19:1 (or maybe other?)

    1 answer

    1. This product is simply a 40% solution of Acrylamide and does not contain any Bis-acrylamide. Note that there are several Acrylamide/Bis-acrylamide 40% solutions available. Please see the link below to review these product options:
      https://www.sigmaaldrich.com/substance/acrylamidebisacrylamide40solution1234598765

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  4. What is the solvent in this solution. Water? Or organic solvent?

    1 answer

    1. The solution is prepared from electrophoresis grade acrylamide in ultra-pure water and aseptically filtered to insure the highest quality and reproducibility.

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