73677

4-Nitrophenyl β-D-glucuronide

β-glucuronidase substrate, chromogenic, ≥99.0% (TLC), powder or crystals, suitable as substrate for β-glucuronidase test

• Synonym(s): 4-Nitrophenyl-β-D-glucopyranosiduronic acid, PNPG
• Empirical Formula (Hill Notation): C₁₂H₁₃NO₉
• CAS Number: 10344-94-2
• Molecular Weight: 315.23
• Beilstein: 1438576
• EC Number: 233-753-0
• MDL number: MFCD00036210
• UNSPSC Code: 12352204
• PubChem Substance ID: 57652486
• NACRES: NA.32

Properties

• product name: 4-Nitrophenyl β-D-glucuronide, ≥99.0% (TLC)
• Quality Level: 200
• Assay: ≥99.0% (TLC)
• form: powder or crystals
• optical activity: [α]20/D −102±3°, c = 1% in ethanol
• impurities: ≤0.05% free 4-nitrophenol; ≤7% water
• solubility: H₂O: 0.1 g/mL, clear, faintly yellow
• suitability: suitable as substrate for β-glucuronidase test
• storage temp.: −20°C
• SMILES string: O[C@@H]1[C@@H](O)[C@@H](O[C@@H]([C@H]1O)C(O)=O)Oc2ccc(cc2)[N+]([O-])=O
• InChI: 1S/C12H13NO9/c14-7-8(15)10(11(17)18)22-12(9(7)16)21-6-3-1-5(2-4-6)13(19)20/h1-4,7-10,12,14-16H,(H,17,18)/t7-,8-,9+,10-,12+/m0/s1
• InChI key: QSUILVWOWLUOEU-GOVZDWNOSA-N

Description

Application

4-Nitrophenyl β-D-glucuronide has been used for determining the β-glucuronidase activity in urine samples, β-glucuronidase activity of bacteria in caecum content digesta and bovine liver. It has also been used to study the inhibitory effect of Phyllanthus amarus extracts on β-glucuronidase.

Substrates

Chromogenic substrate for β-glucuronidase (GUS) gene detection.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves