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391A

Sigma-Aldrich

Leukocyte Peroxidase (Myeloperoxidase) Kit

DAB substrate kit

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About This Item

MDL number:
MFCD00131641
UNSPSC Code:
12352106
NACRES:
NA.47

usage

sufficient for 10-120 manual assays

shelf life

Expiry date on the label.

IVD

for in vitro diagnostic use

application(s)

hematology
histology

shipped in

wet ice

storage temp.

2-8°C

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General description

Used to demonstrate polymorphonuclear cells in blood or bone marrow films. Each kit has sufficient reagents for 10 uses.

Application

Diaminobenzidine, as a benzidine substitute for myeloperoxidase, has less tinctorial power than benzidine. This made DAB much less attractive to histochemists. Hanker and associates improved DAB methodology. According to their modification, the brown reaction product is first intensified with copper salts and followed by application of Gill′s modified Papanicolaou stain, resulting in intense grey-black staining at sites of neutrophil and monocyte peroxidase.
The Leukocyte Peroxidase (Myeloperoxidase) kit is intended for use as a general laboratory histochemical stain for the demonstration of leukocyte peroxidase. This manual, qualitative procedure identifies leukocyte peroxidase in blood or bone marrow films of human specimens.

Kit Components Only

Product No.
Description
  • Diaminobenzidine 10 vial(s)
  • Copper nitrate 2 vial(s)
  • Gill Modified EA solution 100 mL
  • Glutaraldehyde solution 2 x 75
  • Hematoxylin Solution, Gill No. 3 100 mL
  • Scott's Tap Water Substitute Concentrate 100 mL
  • TRIZMAL™ Buffer Concentrate 50 mL

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Muta. 2 - Ox. Liq. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT RE 2 Oral - STOT SE 1 - STOT SE 3

Target Organs

Eyes,Central nervous system, Kidney, Respiratory system

Supplementary Hazards

EUH071

Storage Class Code

5.1A - Strongly oxidizing hazardous materials

WGK

WGK 3

Flash Point(F)

57.2 °F

Flash Point(C)

14 °C

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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