ABD29

Anti-Twist1 (Twist-related protein 1) Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Twist-related protein 1, Class A basic helix-loop-helix protein 38, bHLHa38, H-twist
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ICC, IHC
• Citations: 14

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, rhesus macaque, mouse, horse
• species reactivity (predicted by homology): canine (based on 100% sequence homology), pig (based on 100% sequence homology), equine (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), rhesus monkey (based on 100% sequence homology), gorilla (based on 100% sequence homology)
• packaging: antibody small pack of 25 μg
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin)
• NCBI accession no.: NP_000465
• UniProt accession no.: Q15672
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... TWIST1(7291)

Description

General description

21 kDa calculated

Twist1 is the basic helix-loop-helix transcription factor that regulates embryonic progenitor and transformed tumor cell proliferation, migration and differentiation and is also important in limb development. It has been shown that twist1 expression is high in patients suffering from rheumatic inflammatory disorders with chronically activated T helper lymphocytes and may be used as a biomarker for chronic t-cell activation. Mutations in twist1 are often associated with craniofacial and limb abnormalities in humans with Saethre-Chotzen syndrome. Twist1 dimer affinity can modify the DNA binding affinity. Twist1 has also been implicated as a promoter of tumor growth, invasion and anti-cancer-drug resistance.

Immunogen

Epitope: Near N-terminus

KLH-conjugated linear peptide corresponding to human Twist1 near the N-terminus.

Application

Detect Twist1 (Twist-related protein 1) using this Anti-Twist1 (Twist-related protein 1) Antibody validated for use in IH(P) & IC.

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Twist1 in NIH/3T3, A431, and HeLa cells.

Research Category
Stem Cell Research

Research Sub Category
Transcription Factors

Biochem/physiol Actions

Other homologies: Rat (92% sequence homology).

This antibody recognizes Twist1 near the N-terminus.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Ductal breast fibroadenoma and stratified squamous epithelium and cartilage of human trachea tissues

Evaluated by Immunohistochemistry in ductal breast fibroadenoma and stratified squamous epithelium and cartilage of human trachea tissues.

Immunohistochemistry Analysis: 1:500 dilution of this antibody detected Twist1 in ductal breast fibroadenoma and stratified squamous epithelium and cartilage of human trachea tissues.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable