06-549
Anti-Myc Tag Antibody
Upstate®, from rabbit
Synonym(s):
Transcription factor p64, avian myelocytomatosis viral oncogene homolog, myc proto-oncogene protein, v-myc avian myelocytomatosis viral oncogene homolog, v-myc myelocytomatosis viral oncogene homolog (avian)
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
packaging
antibody small pack of 25 μg
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
General description
Myc proto-oncogene protein (UniProt: P01106; also known as Class E basic helix-loop-helix protein 39 (bHLHe39), Proto-oncogene c-Myc, Transcription factor p64) is encoded by the MYC (also known as BHLHE39) gene (Gene ID: 4609) in human. The Myc-tag is a short peptide sequence derived from the c-Myc protein. It is commonly used as an epitope tag in molecular biology. It is composed of 10 amino acids (EQKLISEEDL) and is fused to proteins of interest to facilitate their detection and purification. The Myc-tag functions as a marker that can be recognized by specific antibodies, allowing researchers to track the expression, localization, and interaction of tagged proteins in various experimental contexts.
Specificity
Other species cross-reactivity not tested.
This rabbit polyclonal antibody detects human Myc and Myc-tagged recombinant proteins.
Immunogen
Epitope: a.a. 409-420
Synthetic peptide corresponding to residues 409-420 of human Myc (MEQKLISEEDLN).
Application
Anti-Myc Tag, Cat. No. 06-549, is a rabbit polyclonal antibody that detects Myc-tagged proteins and is tested for use in Western Blotting and Immunocytochemistry.
Research Category
Epitope Tags & General Use
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Epitope Tags
Tested Applications Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected Myc-tagged PP2A A subunit in NIH/3T3 cells transfected with Myc-tagged PP2A A subunit. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Quality
Evaluated by Western Blotting in NIH/3T3 cells transfected with a Myc-tagged PP2A A subunit. Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected the Myc-tag in NIH/3T3 cells transfected with Myc-tagged PP2A A subunit.
Target description
Predicted molecular weight for human Myc: 49 kDa Obeserved molecular weight may vary.
Physical form
Format: Purified
Protein A chromatography
Purified rabbit in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide. Frozen solution.
Storage and Stability
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Positive control for human Myc: widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.
Positive control for human Myc: widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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