04-1570-I
Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7) Antibody, clone 4E12
culture supernatant, clone 4E12, from rat
Synonym(s):
DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rat
Quality Level
antibody form
culture supernatant
antibody product type
primary antibodies
clone
4E12, monoclonal
species reactivity
mouse
species reactivity (predicted by homology)
human (based on 100% sequence homology)
technique(s)
ChIP: suitable
ELISA: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer7)
Gene Information
human ... POLR2B(5431)
General description
RPB1 (RNA polymerase II subunit B1) is the catalytic component of RNA polymerase II which synthesizes mRNA and non-coding RNAs. During transcription, elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of RPB1, which acts as an assembly platform for factors regulating transcription initiation, elongation, termination and mRNA processing. Phosphorylation occurs mainly at residues ′Ser-2′ and ′Ser-5′ of the tandem 7 residue repeats in the C-terminal domain (CTD), which activates Pol II. This phosphorylation also can occur at ′Ser-7′ of the heptapepdtide repeat. This antibody recognizes the ′Ser-7′ CTD residue of RPB1.
Specificity
Demonstrated to react with human RBP1 in Ni et al., 2011. Transcription 2:5, 237-242
This antibody recognizes RNA polymerase II subunit B1 phosphorylated at Ser7 of the C-terminal domain (CTD).
Immunogen
Epitope: Phosphorylated Ser7 of the C-terminal domain (CTD)
Ovalbumin-conjugated linear peptide corresponding to human RNA polymerase II subunit B1 phosphorylated at Ser7 of the C-terminal domain (CTD).
Application
Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7) Antibody, clone 4E12 is a highly specific rat monoclonal antibody & that targets RNA Polymerase & has been tested in western blotting.
Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to immunoprecipitate RNA polymerase II subunit B1 (phospho-CTD Ser-5) in ChIP (Chapman, R., et al. (2007). Science. 318(5857):1780-1782.).
Demonstrated to react with human RBP1 in Ni et al., 2011. Transcription 2:5, 237-242
Demonstrated to react with human RBP1 in Ni et al., 2011. Transcription 2:5, 237-242
Quality
Evaluated by Western Blotting in untreated and lambda phosphatase treated NIH/3T3 cell lysate.
Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected RNA polymerase II subunit B1 (phospho-CTD Ser-7) in 10 μg of untreated NIH/3T3 cell lysate.
Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected RNA polymerase II subunit B1 (phospho-CTD Ser-7) in 10 μg of untreated NIH/3T3 cell lysate.
Target description
~220 kDa observed
Linkage
Replaces: 04-1570
Physical form
Rat monoclonal IgG1κ with 0.05% sodium azide.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
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Rob D Chapman et al.
Science (New York, N.Y.), 318(5857), 1780-1782 (2007-12-15)
RNA polymerase II is distinguished by its large carboxyl-terminal repeat domain (CTD), composed of repeats of the consensus heptapeptide Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Differential phosphorylation of serine-2 and serine-5 at the 5' and 3' regions of genes appears to coordinate the localization of
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