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U4754

Sigma-Aldrich

Anti-Lectin, Ulex europaeus-I antibody produced in rabbit

whole antiserum, delipidized

Synonym(s):

Anti-Gorse or Furze Lectin, Anti-UEA-I

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About This Item

MDL number:
MFCD00162898
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

15 mM sodium azide

species reactivity

Ulex europaeus

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
indirect immunofluorescence: 1:500 using human tissue

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

The antibody may be used for studies of the vascular structures. It reacts specifically with UEA-I bound to human endothelial cells of normal and neoplastic blood and lymphatic vessels. Its uniform reaction with vessels enables differentation between vessels and artificial slits in the tissue.

Immunogen

Ulex europaeus from gorse seeds..

Application

Anti-Lectin, Ulex europaeus-I antibody produced in rabbit has been used in double immunostaining of muscle capillary sections.

Biochem/physiol Actions

Lectin isolated from Ulex europaeus I is specific for α-L-fucose-containing glycocompounds. Due to this specificity it serves as a marker for vascular endothelial cells and tumors derived from them.

Target description

Lectins are highly specific carbohydrate binding proteins useful in carbohydrate and cell membrane studies, as well as cell agglutination and typing techniques.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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H Holthöfer et al.
Laboratory investigation; a journal of technical methods and pathology, 47(1), 60-66 (1982-07-01)
Ulex europaeus I agglutinin, a lectin specific for some alpha-L-fucose-containing glycocompounds, was used in fluorescence microscopy to stain cryostat sections of human tissues. The endothelium of vessels of all sizes was stained ubiquitously in all tissues studied as judged by
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Some skin and soft tumors, which generally are assumed to be derived from endothelial cells or blood vessels, were characterized with fluorochrome-labeled Ulex europaeus I agglutinin (UEA I), recently shown to bind specifically to endothelial cells in various normal human
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