SRP3204
IL-12 from mouse
recombinant, expressed in CHO cells, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym(s):
CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), NKSF, TSF
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
Recommended Products
biological source
mouse
recombinant
expressed in CHO cells
Assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
potency
≤0.1 ng/mL ED50
mol wt
40 kDa
packaging
pkg of 10 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
shipped in
wet ice
storage temp.
−20°C
Gene Information
mouse ... IL12A(16159)
General description
IL12 (interleukin 12), a member of IL-12 family, is an immunoregulatory cytokine. It is a heterodimer composed of p40 and p35 subunit linked covalently by disulfide bonds. It has a molecular weight of 70kDa. p40 subunit is copiously produced by phagocytic cells, whereas p35 is produced at low level ubiquitously and constitutively. This cytokine was first recognized in 1989 as a natural killer (NK) cell-stimulatory factor. It is predominantly expressed on antigen-presenting cells (APCs) including dendritic cells (DCs), monocytes, macrophages, and Toll-like receptor induced-B cells.
Recombinant murine IL-12 is a 75.0 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (506 total amino acid residues).
Recombinant murine IL-12 is a 75.0 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (506 total amino acid residues).
Biochem/physiol Actions
IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-g production by NK and T cells. Recombinant murine IL-12 is a 75.0 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (506 total amino acid residues).
IL12 (interleukin 12) is crucial for the maturation of naive T cells into IFNγ (interferon)–producing Th1 (T helper) cells. IL12 mutant mice show higher susceptibility to bacterial infections due to significant reduction in the production of IFNγ. This protein functions as an early pro-inflammatory cytokine during infections. IL12 has potent anti-tumor effects in preclinical studies, which however, have not been reproduced in clinical studies. Studies in mice show that IL12 plays a crucial role in immune response against methicillin-resistant Staphylococcus aureus (MRSA)-mediated lung infection. Mice treated exogenously with IL12 prior to being subjected to MRSA infection show enhanced bacterial clearance and non-fatality.
Sequence
p35 Subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA p40 Subunit: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS
Physical form
Lyophilized from 0.5x PBS, pH 7.2.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in 1x PBS, pH 7.2-7.4 to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Marine Cazaux et al.
The Journal of experimental medicine, 216(5), 1038-1049 (2019-04-03)
CAR T cells represent a potentially curative strategy for B cell malignancies. However, the outcome and dynamics of CAR T cell interactions in distinct anatomical sites are poorly understood. Using intravital imaging, we tracked interactions established by anti-CD19 CAR T
New insights into IL-12-mediated tumor suppression.
Tugues S et al
Cell Death and Differentiation, 22(2) , 237-246 (2015)
Role of Interleukin-12 in Protection against Pulmonary Infection with Methicillin-Resistant Staphylococcus aureus.
Nguyen QT et al
Antimicrobial Agents and Chemotherapy, 59(10), 6308-6316 (2015)
Roxana Khazen et al.
The EMBO journal, 40(11), e106658-e106658 (2021-04-16)
Cytotoxic T cells (CTLs) can eliminate tumor cells through the delivery of lethal hits, but the actual efficiency of this process in the tumor microenvironment is unclear. Here, we visualized the capacity of single CTLs to attack tumor cells in
B Oppmann et al.
Immunity, 13(5), 715-725 (2000-12-15)
A novel sequence discovered in a computational screen appears distantly related to the p35 subunit of IL-12. This factor, which we term p19, shows no biological activity by itself; instead, it combines with the p40 subunit of IL-12 to form
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