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RAB0173

Sigma-Aldrich

Human ErbB2 / HER2 ELISA Kit

for serum, plasma, cell culture supernatant and urine

Synonym(s):

ERBB2, HER2, Receptor tyrosine-protein kinase erbB-2, Tyrosine kinase-type cell surface receptor HER2

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.77

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type urine
sample type plasma
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 8 pg/mL
standard curve range: 8.19-2000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... ERBB2(2064)

General description

ERBB2 (erb-b2 receptor tyrosine kinase 2) is a tyrosine kinase receptor that belongs to the ErbB family. It is also known as human epidermal growth factor receptor 2 (HER2)/Neu and is located on human chromosome 17q12. The human ErbB2 (epidermal factor growth factor receptor 2) ELISA (enzyme-linked immunosorbent assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ErbB2 in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human ErbB2 / HER2

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

ERBB2 (erb-b2 receptor tyrosine kinase 2) is considered as a therapeutic target in breast cancer. It controls the concentration of intracellular calcium in SKBR3 (breast cancer cell line) cells.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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HER2 signaling regulates HER2 localization and membrane retention
Jeong J, et al.
PLoS ONE, 12(4), e0174849-e0174849 (2017)
Detection and mapping of amplified DNA sequences in breast cancer by comparative genomic hybridization
Kallioniemi A, et al.
Proceedings of the National Academy of Sciences of the USA, 91(6), 2156-2160 (1994)
The HER2 phenotype of circulating tumor cells in HER2-positive early breast cancer: A translational research project of a prospective randomized phase III trial
Jaeger BAS, et al.
PLoS ONE, 12, e0173593-e0173593 (2017)

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