39220

Dansyl chloride

suitable for fluorescence, BioReagent, ≥99.0% (HPLC)

• Synonym(s): 1-Dimethylaminonaphthalene-5-sulfonyl chloride, 5-(Dimethylamino) naphthalene-1-sulfonyl chloride, DNS Chloride, DNSCl, 5-(Dimethylamino)naphthalene-1-sulfonyl chloride
• Empirical Formula (Hill Notation): C₁₂H₁₂ClNO₂S
• CAS Number: 605-65-2
• Molecular Weight: 269.75
• Beilstein: 2217205
• EC Number: 210-092-6
• MDL number: MFCD00003985
• UNSPSC Code: 12352101
• PubChem Substance ID: 329755828
• NACRES: NA.32

Properties

• product line: BioReagent
• Quality Level: 200
• Assay: ≥99.0% (HPLC)
• mp: 72-74 °C (lit.)
• solubility: acetone: 0.2 g/10 mL
• fluorescence: λ_ex 337 nm; λ_em 492 nm in chloroform (after derivatization with hexylamine)
• suitability: suitable for fluorescence
• storage temp.: 2-8°C
• SMILES string: CN(C)c1cccc2c(cccc12)S(Cl)(=O)=O
• InChI: 1S/C12H12ClNO2S/c1-14(2)11-7-3-6-10-9(11)5-4-8-12(10)17(13,15)16/h3-8H,1-2H3
• InChI key: XPDXVDYUQZHFPV-UHFFFAOYSA-N

Description

General description

Dansyl Chloride, also known as 5-dimethylamino naphthalene-1-sulfonyl chloride, is a pre-column derivatization reagent. After the derivatization reaction, the fluorescent derivatives are analyzed using several analytical methods like NMR, IR, UV-vis, and fluorescence microscopy. Dansyl Chloride is a typical aromatic sulphonyl chloride that reacts with various bases, resulting in derivatives with variable stability.

Application

Dansyl Chloride is used as a derivatization reagent to study extensive metabolism and low doses of ethinylestradiol administered during pre-clinical studies. Studies conducted to determine bisphenols in human serum through the HPLC method used Dansyl Chloride to obtain the derivative products. Dansyl Chloride derivatives are suitable for LC/MS determination and characterization of glyphosate, aminomethylphosphonic acid (AMPA), and glufosinate from food samples.

A fluorogenic reagent for fluorescent N-terminal derivatization of amino acids and peptides and detection by reverse phase HPLC.

Safety Information

• Pictograms: GHS05
• Signal Word: Danger
• Hazard Statements: H290,H314
• Precautionary Statements: P260 - P280 - P303 + P361 + P353 - P305 + P351 + P338 + P310
• Hazard Classifications: Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves