219466

CELLULYSIN^® Cellulase, Trichoderma viride

• Synonym(s): β-D-glucosidase, endo-β-1,4 glucanase, cellobiohydrolase
• CAS Number: 9012-54-8
• Enzyme Commission number: 3.2.1.4 (BRENDA, IUBMB)
• MDL number: MFCD00081510
• UNSPSC Code: 12352202
• NACRES: NA.54

Properties

• biological source: fungus (Trichoderma viride)
• form: solid
• specific activity: ≥10,000 units/g dry wt.
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• technique(s): transformation: suitable
• color: yellow-white
• solubility: water: soluble
• suitability: suitable for molecular biology
• application(s): food and beverages; pharmaceutical
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Cellulase includes endo-β-1,4 glucanase, cellobiohydrolase, and β-D-glucosidase. It is commonly produced by bacteria and fungi.

Application

CELLULYSIN^® Cellulase, Trichoderma viride has been used:

• to digest transgenic BY-2 cells exposed to paraformaldehyde fixation
• in the enzyme mix used for in situ hybridization to plant chromosomes
• in the enzyme solution for the transformation of Phytophthora species
• to isolate intact cells and protoplasts from plant tissues

Biochem/physiol Actions

Cellulase is useful in food, feed, textile, leather, detergent, and paper industries. It is also applied in fiber modification, biomass fermentation, and pharmaceutical industries.

Warning

Toxicity: Harmful (C)

Unit Definition

One unit is a measure of decomposing activity using a filter paper substrate at 40°C, pH 4.0.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 days at 4°C or for up to 1 month at -20°C.

Other Notes

Takebe, I., et al. 1968. Plant Cell Physiol.9, 115.
Tomita, Y., et al. 1968. J. Ferment. Technol.46, 701.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

CELLULYSIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P304 + P340 + P312 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable