07-1420
Anti-VEGF Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Vascular permeability factor
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, human
technique(s)
ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... VEGFA(7422)
General description
VEGF (Vascular endothelial growth factor, VEGFA, VEGF-A), a dimeric ligand, is among the most potent angiogenic mitogens. VEGF is secreted by tumor cells and other cells exposed to hypoxia. VEGF is a highly specific mitogen for vascular endothelial cells. Seven VEGF isoforms (a, b, c, d, e, f, g) are generated as a result of alternative splicing from a single VEGF gene. The most commonly studied isoforms are VEGF121, VEGF165, and VEGF189 (f, d and b, respectively) All seven isoforms differ in their molecular mass and in biological properties such as their ability to bind to cell-surface heparan-sulfate proteoglycans. The expression of VEGF is potentiated and is secreted by tumor cells in response to hypoxia, by activated oncogenes, and by a variety of cytokines. VEGF induces angiogenesis as well as permeabilization of blood vessels, and plays a central role in the regulation of vasculogenesis. VEGF expression correlates to the degree of tumor vascularization and with increased metastatic risk. Additionally, VEGF induces endothelial cell proliferation, promotes cell migration, and inhibits apoptosis. Consequently, inhibition of VEGF signaling abrogates the development of a wide variety of tumors.
Specificity
Recognizes Vascular Endothelial Growth Factor (VEGF), a secreted homodimeric protein secreted by a variety of vascularized tissues. The reported activities of VEGF include stimulation of endothelial cell growth, angiogenesis, and capillary permeability.
Immunogen
Highly pure (>98%) recombinant mouse Vascular Endothelial Growth Factor.
Application
Anti-VEGF Antibody detects level of VEGF & has been published & validated for use in WB, IH(P), ELISA.
Immunohistochemisty (paraffin): VEGF staining in mouse placenta, tissue was pretreated with citrate buffer, pH 6.0. A previous lot of antibody was diluted to 1:100, using IHC-Select detection with HRP-DAB.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mouse VEGF (50.0 ng/mL), a concentration of 1.0-2.0 µg/mL of a previous lot of this antibody was required.
ELISA: A concentration of at least 0.5 µg/mL (100 μL/well) is required to detect 0.2-0.4 ng/well of recombinant mouse VEGF using a previous lot of this antibody.
Optimal working dilutions must be determined by the end user.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mouse VEGF (50.0 ng/mL), a concentration of 1.0-2.0 µg/mL of a previous lot of this antibody was required.
ELISA: A concentration of at least 0.5 µg/mL (100 μL/well) is required to detect 0.2-0.4 ng/well of recombinant mouse VEGF using a previous lot of this antibody.
Optimal working dilutions must be determined by the end user.
Research Category
Signaling
Signaling
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
Quality
Routinely evaluated by Western Blot on Huvec lysates.
Western Blot Analysis:
1:500 dilution of this lot detected VEGF on 10 μg of Huvec lysates.
Western Blot Analysis:
1:500 dilution of this lot detected VEGF on 10 μg of Huvec lysates.
Target description
27 kDa
Linkage
Replaces: AB2142P
Physical form
Affinity purified
Affinity purified rabbit polyclonal IgG employing immobilized mouse VEGF matrix. Antibody solution (1 mg/ml) was lyophilized from PBS, pH 7.2. Reconstitute with sterile distilled water.
Storage and Stability
Reconstituted antibody, at a concentration of ≥ 0.2 mg/mL, is stable for at least six weeks at 2-8ºC. Aliquots stored at -20ºC are stable for at least one year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Huvec cell lysate
Huvec cell lysate
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Zhezhi Deng et al.
Cell cycle (Georgetown, Tex.), 16(8), 785-794 (2017-03-16)
Salt (sodium chloride, NaCl) accumulation in the brain is associated with various diseases of central nervous system (CNS). Activation of astrocytes is an important manifestation of pathophysiological processes in the CNS. However, the direct impact of high salt (HS) environment
Zsuzsanna Lichner et al.
The American journal of pathology, 180(4), 1675-1687 (2012-02-14)
The von Hippel-Lindau (VHL) gene is lost in ≈ 70% of all renal cell carcinomas (RCCs); however, increasing evidence supports the involvement of alternative mechanisms in the regulation of VHL expression, including suppression by microRNAs (miRNAs). miRNAs are small, noncoding
Zeqi Zheng et al.
Cell cycle (Georgetown, Tex.), 17(8), 1026-1033 (2018-06-19)
The potential mechanism of miRNA released from adipose-derived stem cell (ADSC)-derived micro vesicle (MV) onthe modulation of proliferation, migration and invasion of endothelial cells were explored. In this study, miR-210 level was detected by qT-PCR. Alix, VEGF and RUNX3 expressions
Matthew B Greenblatt et al.
The Journal of experimental medicine, 212(8), 1283-1301 (2015-07-22)
Physiological bone remodeling requires that bone formation by osteoblasts be tightly coupled to bone resorption by osteoclasts. However, relatively little is understood about how this coupling is regulated. Here, we demonstrate that modulation of NF-κB signaling in osteoclasts via a
Zhen-Kun Liu et al.
PloS one, 7(9), e45224-e45224 (2012-10-03)
Previously we have screened out Insulin-like Growth Factor Binding Protein 7 (IGFBP7) as a differentially expressed gene in post-implantation uterus versus pre-implantation uterus by suppressive subtractive hybridation. However its function in uterus was not clearly identified. In this research, the
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service