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Key Documents

T1949

Sigma-Aldrich

Total Protein Reagent

Synonym(s):

Biuret Reagent

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About This Item

UNSPSC Code:
12352202
NACRES:
NC.07

Application

Total Protein Reagent can be used for the quantitative, colorimetric determination of total protein concentration in solution at 540 nm. The total protein reagent is supplied as a ready-to-use liquid.
Total Protein Reagent has been used in the biuret assay.

Biochem/physiol Actions

A biuret-based total protein reagent. Copper ions in the alkaline reagent react with peptide bonds in proteins resulting in a purple color with an absorbance maximum of 540 nm.

Pictograms

CorrosionEnvironment

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B

Storage Class Code

8B - Non-combustible corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Determination of serum proteins by means of the biuret reagent.
Gornall, A. G., et al.,
The Journal of Biological Chemistry, 177, 759-759 (1949)
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