Skip to Content
Merck
All Photos(2)

Documents

P2871

Sigma-Aldrich

Anti-Cytokeratin pan antibody, Mouse monoclonal

clone C-11, purified from hybridoma cell culture

Synonym(s):

Anti-CK-4, Anti-CK4, Anti-CYK4, Anti-K4, Anti-WSN1

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

C-11, monoclonal

form

buffered aqueous solution

technique(s)

immunocytochemistry: 15-30 μg/mL using methanol/acetone-fixed PTK-2 cells or A549 - human lung carcinoma cells
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 15-30 μg/mL using human skin
immunohistochemistry (frozen sections): suitable
microarray: suitable
western blot: suitable

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Monoclonal Anti-Cytokeratin, pan (mouse IgG1 isotype) is derived from the C-11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a keratin-enriched preparation from cultured human epidermoid carcinoma cell line A431. Cytokeratin is a member of the intermediate filaments family.

Application

Monoclonal Anti-Cytokeratin pan antibody has been used in immunoblotting, dot blot, immunohistochemistry, immunocytochemistry, immunofluorescence, immunostaining, detecting micro-metastases in lymph nodes, bone marrow, and other tissues and for determining the origin of poorly differentiated tumors.

Biochem/physiol Actions

Cytokeratin plays a major role in observing carcinomas.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Co-culture with endometrial stromal cells enhances the differentiation of human embryonic stem cells into endometrium-like cells
Yu W, et al.
Experimental and Therapeutic Medicine, 10(1), 43-50 (2015)
Aminah Ali Abid Al-Juboori et al.
Proteomics, 19(21-22), e1800448-e1800448 (2019-03-14)
Epithelial and stromal communications are essential for normal uterine functions and their dysregulation contributes to the pathogenesis of many diseases including infertility, endometriosis, and cancer. Although many studies have highlighted the advantages of culturing cells in 3D compared to the
Utero-placental expression of angiotensin-(1-7) and ACE2 in the pregnant guinea-pig
Valdes G, et al.
Reproductive Biology and Endocrinology, 11(1), 5-5 (2013)
Caspase-cleaved cytokeratin 18 fragment (M30) as marker of postoperative residual tumor load in colon cancer patients
Ausch C, et al.
European Journal of Surgical Oncology, 35(11), 1164-1168 (2009)
Spatio-temporal expression of MMP-2, MMP-9 and tissue kallikrein in uteroplacental units of the pregnant guinea-pig (Cavia porcellus)
Corthorn J, et al.
Reproductive Biology and Endocrinology, 5(1), 27-27 (2007)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service