MSST0002
SILu™Lite APOA1 Apolipoprotein A-1 human
recombinant, expressed in HEK 293 cells, MS Protein Standard
Synonym(s):
SILuLite Apolipoprotein A-1
About This Item
Recommended Products
biological source
human
Quality Level
recombinant
expressed in HEK 293 cells
tag
His tagged
V5 tagged
Assay
≥98% (SDS-PAGE)
form
lyophilized powder
packaging
vial of 50-65 μg (Lot-specific vial content given on certificate of analysis)
technique(s)
mass spectrometry (MS): suitable
UniProt accession no.
storage temp.
−20°C
Related Categories
1 of 4
This Item | HPA030924 | HPA043513 | HPA014917 |
---|---|---|---|
affinity isolated antibody | affinity isolated antibody | affinity isolated antibody | affinity isolated antibody |
unconjugated | unconjugated | unconjugated | unconjugated |
Prestige Antibodies® Powered by Atlas Antibodies | Prestige Antibodies® Powered by Atlas Antibodies | Prestige Antibodies® Powered by Atlas Antibodies | Prestige Antibodies® Powered by Atlas Antibodies |
human ... C2CD4C(126567) | human ... C2CD2(25966) | human ... CARD8(22900) | human ... DENND4C(55667) |
polyclonal | polyclonal | polyclonal | polyclonal |
Biochem/physiol Actions
Physical form
Analysis Note
SILuLite APOA1 is a recombinant human protein expressed in human 293 cells. It is a monomer of 286 amino acids (including N-terminal signal sequence and C-terminal polyhistidine and V5 tags), with a molecular weight of 32.1 kDa. SILuLite APOA1 is an analytical standard designed to be used as starting material for preparation of calibrators and controls in LC-MS applications.
Sequence
RHFWQQDEPPQSPWDRVKDLATVYVDVLKDSGRDYVSQFEGSALGKQLNLKLLDNWDSVTSTFSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQEKLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEYHAKATEHLSTLSEKAKPALEDLRQGLLPVLESFKVSFLSALEEYTKKLNTQSDPSRGPFEGKPIPNPLLGLDSTRTGHHHHHHHHGGQ
The N-terminal signal peptide and C-terminal linker peptide, V5 and polyhistidine tags are italicized.
Other Characterization
- Sequence confirmed by intact mass analysis
- Identity verified by peptide mapping
- purity >98% (SDS-PAGE)
- Vial content was determined by the Bradford method using BSA as a calibrator. The correction factor of Bradford-to-AAA is 88.33%
Suggested Quantitative Analysis Parameters
(MRM settings provided for three suggested peptides)
Legal Information
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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