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Merck

MSST0002

Sigma-Aldrich

SILuLite APOA1 Apolipoprotein A-1 human

recombinant, expressed in HEK 293 cells, MS Protein Standard

Synonym(s):

SILuLite Apolipoprotein A-1

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About This Item

UNSPSC Code:
23201100
NACRES:
NA.12

Pricing and availability is not currently available.

biological source

human

Quality Level

recombinant

expressed in HEK 293 cells

tag

His tagged
V5 tagged

Assay

≥98% (SDS-PAGE)

form

lyophilized powder

packaging

vial of 50-65 μg (Lot-specific vial content given on certificate of analysis)

technique(s)

mass spectrometry (MS): suitable

UniProt accession no.

storage temp.

−20°C

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This Item
HPA030924HPA043513HPA014917
antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

product line

Prestige Antibodies® Powered by Atlas Antibodies

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Prestige Antibodies® Powered by Atlas Antibodies

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Prestige Antibodies® Powered by Atlas Antibodies

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Prestige Antibodies® Powered by Atlas Antibodies

Gene Information

human ... C2CD4C(126567)

Gene Information

human ... C2CD2(25966)

Gene Information

human ... CARD8(22900)

Gene Information

human ... DENND4C(55667)

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

Biochem/physiol Actions

Apolipoprotein A-1(Apo-A1), is a major protein component of high density lipoprotein (HDL)in plasma1. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters2. Apo-A1 was shown to assist in the improvement of predicting cardiovascular events3. The gene that encodes to Apolipoprotein A-1 is APOA1. This gene is closely linked with two other apolipoprotein genes on chromosome 114. Defects in this gene are associated with HDL deficiencies, including Tangier disease5, and with systemic non-neuropathic amyloidosis6. The protein is made up of one major isoform (pI 5.6) and two minor isoforms (pI 5.53 and 5.46). Apo-A1 shows a high content of a-helix structure6. The amphipathic regions in the α-helix structure seem to be responsible for lipid binding capacity. In aqueous solution, Apo-A1 shows self-association with minor conformational change7. In addition, it has been shown that Apo-A1 is implicated in the anti-endotoxin function of HDL via interaction with lipopolysaccharide or endotoxin8.

Physical form

Supplied as a lyophilized powder containing phosphate buffered saline.

Analysis Note

General Description

SILuLite APOA1 is a recombinant human protein expressed in human 293 cells. It is a monomer of 286 amino acids (including N-terminal signal sequence and C-terminal polyhistidine and V5 tags), with a molecular weight of 32.1 kDa. SILuLite APOA1 is an analytical standard designed to be used as starting material for preparation of calibrators and controls in LC-MS applications.

Sequence

RHFWQQDEPPQSPWDRVKDLATVYVDVLKDSGRDYVSQFEGSALGKQLNLKLLDNWDSVTSTFSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQEKLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEYHAKATEHLSTLSEKAKPALEDLRQGLLPVLESFKVSFLSALEEYTKKLNTQSDPSRGPFEGKPIPNPLLGLDSTRTGHHHHHHHHGGQ

The N-terminal signal peptide and C-terminal linker peptide, V5 and polyhistidine tags are italicized.

Other Characterization

  • Sequence confirmed by intact mass analysis
  • Identity verified by peptide mapping
  • purity >98% (SDS-PAGE)
  • Vial content was determined by the Bradford method using BSA as a calibrator. The correction factor of Bradford-to-AAA is 88.33%


Suggested Quantitative Analysis Parameters
(MRM settings provided for three suggested peptides)

Legal Information

SILu is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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This is a sample Certificate of Analysis (COA) and may not represent a recently manufactured lot of this specific product.

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B Lamarche et al.
Clinica chimica acta; international journal of clinical chemistry, 286(1-2), 145-161 (1999-10-08)
Reduced plasma high-density lipoprotein (HDL) cholesterol levels have been recognized as a highly significant independent risk factor for atherosclerotic cardiovascular disease. HDL levels are also inversely related to plasma triglyceride levels and there is a dynamic interaction between HDL and
E M Rubin et al.
Proceedings of the National Academy of Sciences of the United States of America, 88(2), 434-438 (1991-01-15)
In Western societies high density lipoprotein (HDL) levels correlate inversely with the risk for coronary heart disease. The primary protein component of both human and mouse HDL is apolipoprotein A-I (apoAI), which comprises greater than 70% of HDL protein and

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