The iron content for this product ranges from 0.25% to 0.32%. The specific iron content is lot-dependent and can be found in the Certificate of Analysis.
M0630
Myoglobin from equine skeletal muscle
95-100%, essentially salt-free, lyophilized powder
Synonym(s):
Myoglobin from horse skeletal muscle
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About This Item
Form:
essentially salt-free, lyophilized powder
Assay:
95-100%
Biological source:
equine skeletal muscle
Mol wt:
~17 kDa(lit.)
Pricing and availability is not currently available.
biological source
equine skeletal muscle
Quality Segment
assay
95-100%
form
essentially salt-free, lyophilized powder
mol wt
~17 kDa(lit.)
Iron content
0.25-0.32%
technique(s)
mass spectrometry (MS): suitable
solubility
H2O: soluble 10 mg/mL
UniProt accession no.
storage temp.
−20°C
Gene Information
horse ... MB(100054434)
Application
Biochem/physiol Actions
Myoglobin from horse skeletal muscle is a single chain heme protein containing 153 amino acid residues. It possesses no disulfide bridges or free -SH groups. Myoglobin contains 8 variously sized right-handed helical regions, joined by non-ordered or random coil regions.
Myoglobin is critical to skeletal muscle O2 supply at near-maximum oxygen demand, and prevents anoxia by maintaining PO2 above levels needed to support mitochondrial function.
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This Item | |||
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| description 95-100%, essentially salt-free, lyophilized powder | description ≥95% (SDS-PAGE) | description 98-100%, essentially salt-free, lyophilized powder | description ≥95% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal FLAG® tagged), lyophilized powder |
| biological source equine skeletal muscle | biological source horse heart | biological source human | biological source human |
| assay 95-100% | assay ≥95% (SDS-PAGE) | assay 98-100% | assay ≥95% (SDS-PAGE) |
| technique(s) mass spectrometry (MS): suitable | technique(s) cell based assay: suitable | technique(s) cell culture | mammalian: suitable | technique(s) mass spectrometry (MS): suitable |
| mol wt ~17 kDa(lit.) | mol wt 12,384 | mol wt - | mol wt 10 kDa |
| form essentially salt-free, lyophilized powder | form powder | form essentially salt-free, lyophilized powder | form lyophilized powder |
| solubility H2O: soluble 10 mg/mL | solubility H2O: soluble 10 mg/mL | solubility H2O: soluble 10 mg/mL | solubility 0.05 M Tris pH 7.5: ≥10 mg/mL, clear to slightly hazy, colorless |
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Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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Do you know the iron content in total for this product (1g)?
1 answer-
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Can you advise what the % level of hemoglobin (if any will be present in this product)? Or can this be treated as Hb contaminant free.
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While this product is not specifically tested for hemoglobin, it is purified by Sephadex gel filtration. Given the molecular weight differences between these two compounds, the presence of hemoglobin is highly unlikely.
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Can you share the amino acid sequence of this protein? M0630
1 answer-
This product is a native protein that has not been modified during the extraction and purification process. The sequence of this product is published information. Please refer to the information in the UniProt database: https://www.uniprot.org/uniprotkb/P68082/entry
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Is this myoglobin detectable using immunoenzymatic assay?
1 answer-
Unfortunately, none of the equine myoglobin products have been tested for suitability in ELISA. Performance of these products in immunoenzymatic assays cannot be validated.
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