D8144
Dextranase from Penicillium sp.
lyophilized powder, 400-800 units/mg protein
Synonym(s):
1,6-α-D-Glucan 6-glucanohydrolase
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About This Item
Recommended Products
biological source
Penicillium sp.
Quality Level
form
lyophilized powder
specific activity
400-800 units/mg protein
composition
Protein, 35% Lowry
storage temp.
2-8°C
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General description
Dextranase from fungi belong to the glycoside hydrolase families (GH) 49. Dextranase from Penicillium sp. are stable at varying temperature and pH.
Application
Dextranase from Penicillium sp has been used:
- in the digestion of exopolysaccharides from P. aeruginosa biofilms and dextran polymers
- as a component of trypticase soy broth (TSYPS) for Streptococcus mutans culture
- in microgel degradation
Biochem/physiol Actions
An endodextranase that hydrolyzes -(1,6)-glucosidic linkages in dextran. Dextrans are undesirable compounds synthesized from sucrose by microbial contaminants during sugar production that increase viscosity of the flow and decrease industrial recovery. Dextranase has been used for hydrolyzing dextran at sugar mills in order to improve efficiency of sugar production.
Unit Definition
One unit will liberate 1.0 μmole of isomaltose (measured as maltose) per min at pH 6.0 at 37 °C, using dextran as substrate.
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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T I Danilova et al.
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The carbohydrate composition of dextranase from Penicillium funiculosum 15, as well as the composition of products of dextran deep hydrolysis by the enzyme were studied. The products are normally used to stabilize the enzyme during its purification. Using the methods
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