A0457

Anti-Activin Receptor IIB antibody produced in goat

affinity isolated antibody

• Synonym(s): ActRIIB
• UNSPSC Code: 51111800
• NACRES: NA.41
• MDL number: MFCD01864596
• Conjugate: unconjugated
• Clone: polyclonal
• Application: FACS, IHC, WB
• Citations: 4

Properties

• biological source: goat
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human
• technique(s): flow cytometry: 10 μL using 1-2.5 x 10⁵ cells (of a 25 μg/mL dilution), immunohistochemistry: 5 μg/mL, western blot: 0.1-0.2 μg/mL
• UniProt accession no.: Q13705
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ACVR2B(93)

Description

General description

Activin is a disulfide-linked dimeric protein released by grandulosa cells in the ovary and sertoli cells in the testis. Activin Receptor IIB belongs to Ser/Thr protein kinase family and can transduce the activin signal from the cell surface to the cytoplasm. Activin will not bind directly to Type I activin receptors rather it will associate with the type II receptor-activin complex and start signal transduction. Many ligands signal through Activin Receptor IIB can regulate muscle growth. Anti-Activin Receptor IIB antibody can be used in flow cytometry and immunohistochemistry. Goat Anti- Activin Receptor IIB antibody reacts specifically with recombinant human activin receptor IIB. The product has shown no cross-reactivity for human activin receptor IA, recombinant human activin receptor IIA or recombinant human activin receptor IB.

Anti-Activin Receptor IIB recognizes recombinant human activin receptor IIB by methods such as immunoblotting and ELISA. The antibody can inhibit receptor-ligand interaction. In ELISA assays, the antibody can block 50% of the binding of recombinant human activin A to recombinant human activin receptor IIB/Fc chimera. Furthermore, the antibody exhibits 15% cross-reactivity with recombinant human activin receptor I. This antibody shows no cross reactivity with recombinant human activin receptor IA, IIA, and IB using ELISA and immunoblotting methods.

Immunogen

purified, NSO-derived, recombinant human activin receptor IIB extracellular domain.

Application

Anti-Activin Receptor IIB can be used in western blotting (working concentration-0.1-0.2 μg/mL) for detecting human activin receptor IIB.

Goat anti-activin receptor IIB antibody can be used for immunohistochemistry (5 μg/mL) and flow cytometry (10 μL) applications.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 5% trehalose

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 13 - Non Combustible Solids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable