41698

Atto 488 NHS ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

• Synonym(s): Atto 488
• NACRES: NA.32
• UNSPSC Code: 12352108

Properties

• product line: BioReagent
• Quality Segment: 100
• assay: ≥90% (HPLC), ≥90% (degree of coupling)
• form: powder
• mol wt: 686.66 g/mol
• manufacturer/tradename: ATTO-TEC GmbH
• color: orange, red
• λmax: 501-507 nm in methanol: water (1:1) (with 0.1% perchloric acid)
• λ: in methanol: water (1:1) (with 0.1% perchloric acid)
• UV absorption: λ: 501-507 nm A_max
• suitability: suitable for fluorescence
• storage temp.: −20°C
• SMILES string: CN(CCCC(=O)ON1C(=O)CCC1=O)C(=O)C2=CC=CC=C2C3=C4C=CC(=[NH2+])C(=C4OC5=C3C=CC(=C5S(=O)(=O)[O-])N)S(=O)(=O)O
• InChI: InChI=1S/C29H26N4O12S2/c1-32(14-4-7-23(36)45-33-21(34)12-13-22(33)35)29(37)16-6-3-2-5-15(16)24-17-8-10-19(30)27(46(38,39)40)25(17)44-26-18(24)9-11-20(31)28(26)47(41,42)43/h2-3,5-6,8-11,30H,4,7,12-14,31H2,1H3,(H,38,39,40)(H,41,42,43)
• InChI key: QYLVUXZLEBHDMK-UHFFFAOYSA-N

Description

General description

Atto 488 NHS ester is a hydrophilic fluorescent label with high water solubility. The fluorescence activity is excited efficiently at the 480-515nm range. A suitable excitation source for Atto 488 is the 488 nm line of the Argon-Ion laser.

Application

Atto 488 NHS ester is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, and STED. In addition, the dye is used in flow cytometry (FACS) and fluorescence in-situ hybridization (FISH) methods.

Features and Benefits

Characteristic features of the Atto 488 NHS ester are:

• Strong Absorption.
• High Fluorescence quantum yield.
• High Photostability.
• Excellent water solubility.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• ppe: Eyeshields, Gloves, type N95 (US)