28713

LB Broth

NutriSelect^® Plus, from non-animal source(Vegitone), powder, pkg of 500 g, non-sterile, nonselective for Escherichia coli

• Synonym(s): E. coli enrichment broth, LB Broth, Vegitone, Luria Bertani Broth, Vegitone, Vegitone LB Broth
• UNSPSC Code: 41171607
• NACRES: NA.85

Properties

• biological source: non-animal source (Vegitone)
• Quality Level: 200
• sterility: non-sterile
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: sodium chloride, 5.0 g/L ; tryptone (vegetable), 10.0 g/L ; yeast extract, 5.0 g/L
• packaging: pkg of 500 g
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• color: clear to slightly opaque yellow to amber Prepared media; light yellow Dehydrated powder
• final pH: 7.0±0.2 (25 °C)
• application(s): agriculture; food and beverages; life science and biopharma; microbiology; pharmaceutical
• suitability: nonselective for Escherichia coli (Recombinant); nonselective for coliforms

Description

General description

Luria Bertani Broth Vegitone is free of animal derived material. It is used for the cultivation and maintenance of recombinant strains of Escherichia coli and may be used for routine cultivation of not particularly fastidious microorganisms.

Preparation Note

Suspend 20 g in 1000 ml distilled water. Heat to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Dispense as desired.

Storage and Stability

• Store prepared media below 8°C, protected from direct light.

• Store dehydrated powder, in a dry place, in tightly sealed containers at 2-25°C.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves