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Key Documents

MR-070

Sigma-Aldrich

EmbryoMax® Human Tubal Fluid (HTF) (1X), liquid, for Mouse IVF

The EmbryoMax Human Tubal Fluid (HTF) (1X), liquid designed for use with Mouse IVF is available in a 50 mL format and has been optimized and validated for Embryo Culture.

Synonym(s):

HTF Media, Human Tubul Fluid Media

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About This Item

UNSPSC Code:
12352207
eCl@ss:
32160801
NACRES:
NA.75
Pricing and availability is not currently available.

Quality Level

300

form

liquid

manufacturer/tradename

Specialty Media
EmbryoMax®

technique(s)

cell culture | embryo: suitable
cell culture | stem cell: suitable

input

sample type: mouse embryo(s)

Related Categories

General description

The EmbryoMax® Human Tubal Fluid (HTF) (1X), liquid designed for use with Mouse IVF is available in a 50 mL format and has been optimized and validated for embryo Culture.

Application

EmbryoMax® Human Tubal Fluid (HTF) (1X), liquid has been used as an in vitro fertilization (IVF) media for mouse oocytes and embryo.

Storage and Stability

Can be stored at -20°C upto the expiration date on the bottle.


Once thawed it should be used within 2 weeks

Legal Information

EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Embryo Culture Media

Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture

Questions

1–2 of 2 Questions  

  1. Should we supplement BSA to the HTF for embryo culture or can we use it directly use it ?

    1 answer

    1. The product is already supplemented with 4.0 g/L BSA.

      Helpful?

  2. Hi! I've been trying to find the detailed composition of the media and couldn't find it. Could you please let me know if the media is supplemented with BSA or is it needed? What buffer does it uses? Thanks in advance, Best, Maria

    1 answer

    1. The composition of the medium MR-070-D EmbryoMax® is as follows:

      Component g/L
      BSA 4.0
      CaCl2-2H2O 0.3
      Glucose 0.5
      KCl 0.3496
      KH2PO4 0.0504
      MgSO4-7H2O 0.0493
      NaCl 5.9375
      NaHCO3 2.1
      Sodium Lactate 3.42 mg/L
      Sodium Pyruvate 0.0365
      Penicillin G Na Salt 0.075
      Phenol Red 2mg/L
      Streptomycin Sulfate 0.05

      Helpful?

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