70239-M
His•Bind® Purification Kit
Synonym(s):
IMAC, Protein purification
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About This Item
UNSPSC Code:
41106500
NACRES:
NA.56
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General description
His•Bind® purification kit includes pre-tested buffers specifically designed for IDA-based His•Bind Resin, enabling convenient and rapid one-step purification of proteins by metal affinity chromatography. It uses the His•Tag®/His•Bind® technology, that leverages the affinity between neighboring histidine in the His•Tag sequence and immobilized metal ions (such as Ni2+ or Co2+).
Application
His•Bind® Purification Kit has been used for: the purification of N-terminal 6-histidine-tagged aldehyde dehydrogenase isozyme, ALDH1A1 under nondenaturing conditions by nickel-NTA affinity chromatography, the purification of virus binding protein specific for Poliovirus Type 1, the purification of recombinant oE7, huhsp70 and oE7/huhsp70 proteins, to study the effective antitumor efficacy of human hsp70 and HPV16 oE7 fusion protein vaccine, the purification of recombinant exFPR1, MTBHsp70 and MTBHsp70-exFPR1 fusion proteins to study their role in cervical cancer immunotherapy
Features and Benefits
- Includes His•Bind® Resin, His•Bind® buffer kit and chromatography columns.
- Solutions are included for Ni2+ charging, binding, washing, and elution.
- Columns are pre-packed with 1.25 ml bed volume of Ni2+-charged His•Bind Resin.
Components
•10 mlHis•Bind Resin
•1His•Bind Buffer Kit
•pkg/4Chromatography Columns
•1His•Bind Buffer Kit
•pkg/4Chromatography Columns
Warning
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Other Notes
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Legal Information
HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 2 - Carc. 1A Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1C - Skin Sens. 1 - STOT RE 1 - STOT RE 2 Inhalation
Target Organs
respiratory tract irritation
Storage Class Code
3 - Flammable liquids
Flash Point(F)
86.0 °F
Flash Point(C)
30 °C
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
MTBHsp70-exFPR1-pulsed Dendritic Cells Enhance the Immune Response against Cervical Cancer
Cao G, et al.
Journal of Cancer, 10(25), 6364?6373-6364?6373 (2019)
Construction of a Cloning System for the Mass Production of a Virus-Binding Protein Specific for Poliovirus Type 1
Sano D and Omura T
Applied and Environmental Microbiology, 71(5) (2005)
A bioluminescence assay for aldehyde dehydrogenase activity
Duellman SJ, et al.
Analytical Biochemistry, 434(2) (2013)
Human hsp70 and HPV16 oE7 fusion protein vaccine induces an effective antitumor efficacy
Jinbao Zong, et al.
Oncology Reports, 30(1), 407-412 (2013)
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