324699
Elastase Substrate IV, Colorimetric
A specific substrate for pancreatic elastase.
Synonym(s):
Elastase Substrate IV, Colorimetric
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About This Item
Quality Level
Assay
≥95% (HPLC)
form
lyophilized solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
desiccated
color
white to off-white
solubility
ethanol: 1 mg/mL
water: soluble
shipped in
ambient
storage temp.
−20°C
General description
A specific substrate for pancreatic elastase (Km = 100 µM; Kcat/Km = 35,000 s-1 M-1 for rat pancreatic elastase; Km = 30 µM; Kcat/Km = 351,000 s-1 M-1 for porcine pancreatic elastase). Cleavage of substrate can be monitored at ~405 nm.
Suggested protocol: see Largman, C. (1983)
Suggested protocol: see Largman, C. (1983)
A specific substrate for pancreatic elastase (km = 100 µM; kcat/ Km = 35,000 M-1 s-1 for rat pancreatic elastase, Km = 30 µM; kcat/Km = 35,000 M-1 s-1 for porcine). Cleavage of substrate can be monitored at ~405 nm.
Biochem/physiol Actions
Cell permeable: no
Primary Target
A specific substrate for pancreatic elastase
A specific substrate for pancreatic elastase
Product does not compete with ATP.
Reversible: no
km = 100 µM; kcat/ Km = 35,000 M-1 s-1 for rat pancreatic elastase; Km = 30 µM; kcat/Km = 35,000 M-1 s-1 for porcine
Warning
Toxicity: Standard Handling (A)
Sequence
Suc-Ala-Ala-Pro-Abu-pNA (Abu = L-α-Aminobutyric Acid)
Physical form
45 mg D-mannitol and 5 mg substrate. Sold on the basis of substrate content.
Reconstitution
Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.
Other Notes
Largman, L. 1983. Biochemistry22, 3763.
Del Mar, E.G., et al. 1980. Biochemistry19, 468.
Del Mar, E.G., et al. 1980. Biochemistry19, 468.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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