1.50798

Purospher^® RP-18 endcapped (5 µm) HPLC Columns

L × I.D. 125 mm x 3 mm LiChroCART^™ HPLC cartridge, manuCART^™ holder required for use

• Synonym(s): Purospher^® RP-18 endcapped (5 µm) LiChroCART^™ 125-3
• UNSPSC Code: 41115709
• NACRES: SB.52
• Particle size: 5 μm
• Matrix active group: C18 (RP18), C18 bonding phase
• Pore size: 120 Å pore size
• Matrix: fully porous particle

Properties

• Product Name: Purospher^® RP-18 endcapped (5 µm), L × I.D. 125 mm x 3 mm LiChroCART^™ HPLC cartridge, manuCART^™ holder required for use
• material: stainless steel column
• Quality Segment: 100
• agency: suitable for USP L1
• product line: Purospher^®
• feature: endcapped
• analyte functional class(es): antibiotics, explosives, fat soluble vitamins, lipids, nutraceuticals and antioxidants, oils, pigments, sweeteners, water soluble vitamins
• packaging: 1 ea of
• extent of labeling: 18% Carbon loading
• parameter: 250 bar pressure, 65 °C max. temp.
• technique(s): HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)
• column L × I.D.: 12.5 cm × 3 mm
• surface area: 350 m²/g
• matrix: fully porous particle
• matrix active group: C18 (RP18), C18 bonding phase
• particle size: 5 μm
• pore size: 120 Å pore size
• operating pH: 2-7.5
• separation technique: reversed phase
• storage temp.: no temp limit

Description

General description

HPLC cartridge

Purospher^® RP-18 endcapped is a versatile high-purity HPLC column producing excellent peak shapes. The excellent balance of Purospher<TMSYMBOL></TMSYMBOL> RP-18 endcapped in the sum of its chromatographic properties is the key to better separations of complex samples with simpler, neutral eluents. The good chemical stability of Purospher<TMSYMBOL></TMSYMBOL> RP-18 endcapped enables the use of mobile phase conditions at pH 8 for a long period of time. The connection to the HPLC system is being accomplished by use of the manu-CART NT cartrige holder (1.51486).

Application

• Determination of nifedipine in serum of women in preterm labor by high-performance liquid chromatography with diode array detection.: This study utilized Purospher^® RP-18 endcapped (5 µm) columns in the HPLC analysis of nifedipine levels in serum samples from women in preterm labor. The research demonstrated the effectiveness of the Purospher^® RP-18 columns in achieving high resolution and sensitivity, making it a valuable tool for bioanalytical applications in clinical chemistry (Kowalczuk et al., 2006).

Analysis Note

Pressure: ≤ 60 bar
Theoretical Plates (N/m) (Anthracene): ≥ 65000
Symmetry (Anthracene): 0.8 - 1.4
Capacity factor (Anthracene): 4.5 - 6.5

Legal Information

LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany

MANUCART is a trademark of Merck KGaA, Darmstadt, Germany

Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany