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1.04302

Hematoxylin cryst. (C.I. 75290)

for microscopy

Synonym(s):

Hematoxylin cryst. (C.I. 75290)

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About This Item

Empirical Formula (Hill Notation):
C16H14O6
CAS Number:
Molecular Weight:
302.28
UNSPSC Code:
41116130
EC Index Number:
208-237-3
NACRES:
NA.21
MDL number:
eCl@ss:
32039090
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Quality Segment

form

solid

IVD

for in vitro diagnostic use

technique(s)

microbe id | staining: suitable

visual transition interval

5.0-7.2, yellow to violet

mp

140 °C (Elimination of water of crystallisation)

bulk density

500 kg/m3

application(s)

cytology
diagnostic assay manufacturing
hematology
histology

storage temp.

15-25°C

SMILES string

O1C[C@@]2([C@H](c4c1c(c(cc4)O)O)c3c(cc(c(c3)O)O)C2)O

InChI

1S/C16H14O6/c17-10-2-1-8-13-9-4-12(19)11(18)3-7(9)5-16(13,21)6-22-15(8)14(10)20/h1-4,13,17-21H,5-6H2/t13-,16+/m1/s1

InChI key

WZUVPPKBWHMQCE-CJNGLKHVSA-N

General description

Hematoxylin crystals are a dye for the specific staining of DNA in the nuclei. It is used in cytology and histology. Nuclei are stained blue, dark violet to black. Hematoxylins are mixed with trivalent positively charged metal salts and build up the so-called hematoxylin lakes, used for the selective staining of nuclei (DNA) .
It is an IVD registered product and CE certified, thus can be used for clinical diagnostic purposes. For more details, please see instructions for use (IFU).


Pictograms

Exclamation mark

Signal Word

Warning

Hazard Codes

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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Questions

  1. Should this product be stirred or mixed before use? 使用前には、攪拌・混和したほうが良いでしょうか?

    1 answer
    1. This product is supplied as a dry crystalline powder. No mixing is required before weighing the powder. During preparation of the staining solution, stirring is necessary as described in the user guide: For the Gill method, stir for 1 hour at room temperature, then filter before use. For the Harris method, dissolve under heating with stirring, followed by further mixing and filtration. Detailed preparation instructions are available in the user guide below.
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/119/913/104302ug-ms.pdf

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