1.00901

Buffered Peptone Water

ReadyBag^®, according to ISO, FDA-BAM, EP, granular, sachets of 60 × 29 g, sterile; γ-irradiated

• Synonym(s): BPW, Peptone water (buffered), Tryptone Phosphate Water
• UNSPSC Code: 41106213
• NACRES: NA.74

Properties

• Agency: APHA; EP; FDA-BAM; ISO 21528; ISO 22964; according to ISO 6579; according to ISO
• Quality Level: 200
• sterility: sterile; γ-irradiated
• form: granular
• feature: ready-to-use
• packaging: sachets of 60 × 29 g
• manufacturer/tradename: ReadyBag^®
• storage condition: (Protected from light. Tightly closed. Dry)
• technique(s): microbiological culture: suitable
• color: beige
• pH: 7.2 (37 °C, 25 g/L in H₂O)
• solubility: 25.5 g/L
• bulk density: 800 kg/m³
• application(s): food and beverages; industrial qc; microbiology; pathogen testing; pharmaceutical
• storage temp.: 15-25°C
• suitability: Cronobacter spp.; Salmonella spp.; enterobacteriaceae

Description

General description

Buffered Peptone Water broth is rich in nutrients and produces high resuscitation rates for sub-lethally injured bacteria, and enables intense growth. The phosphate buffer system prevents bacterial damage caused by changes in the pH of the medium. Peptone, obtained by the enzymatic digest of casein, acts as a source of carbon, nitrogen, vitamins, and minerals, while sodium chloride maintains the osmotic balance.

The Readybag^® sachets are gamma-irradiated (10-20 kGray). Each sachet contains 29 g of granulated Buffered Peptone Water broth for 125 g test samples, to which 1125 ml of sterile water is added.

Application

Buffered Peptone Water is suitable for the preliminary non-selective enrichment of bacteria, particularly pathogenic Enterobacteriaceae such as Salmonella and Cronobacter, from food and animal feed, water, and other materials. It is also used for the dissolving, suspending, and diluting of test samples from food, animal feed, and other materials.

Legal Information

READYBAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3