06-1398
Anti-CDK5RAP2 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
CDK5 regulatory subunit-associated protein 2, CDK5 activator-binding protein C48, Centrosome-associated protein 215
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All Photos(5)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
technique(s)
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CDK5RAP2(55755)
General description
Also known as Cep215, CDK5RAP2 is involved in a variety of cellular processes. It serves as a potential regulator of CDK5 activity through its interaction with CDK5R1. It acts as a negative regulator of centriole disengagement, which maintains centriole engagement and cohesion. It is involved in the regulation of mitotic spindle orientation. Additionally, this protein mediates spindle checkpoint activation by serving as a transcriptional regulator of the MAD2 and BUBR1 promoter. In conjuction with MAPRE1, CDK5RAP2 promotes microtubule polymerization, bundle formation, and growth at the plus ends. It also plays a role in DNA damage-induced G2 cell cycle checkpoint. CDK5RAP2 is known to be mutated in primary microcephaly, a neuodevelopmental disorder that is characterized by a decrease in brain size.
Specificity
Other Homologies: Rat (83% sequence homology) and Mouse (82% sequence homology).
Immunogen
MBP-tagged recombinant protein corresponding to human CDK5RAP2.
Application
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected CDK5RAP2 in RPE1 cells. (Barr, A.R., et al. (2010). J Cell Biol. 189(1):23-39.)
Use Anti-CDK5RAP2 Antibody (rabbit polyclonal antibody) validated in WB, ICC to detect CDK5RAP2 also known as CDK5 regulatory subunit-associated protein 2, CDK5 activator-binding protein C48, Centrosome-associated protein 215.
Quality
Evaluated by Western Blot in 10 µg of HeLa cell lysate.
Western Blot Analysis: A 1:2,500 dilution of this antibody dectected CDK5RAP2 in 10µg of HeLa cell lysate.
Western Blot Analysis: A 1:2,500 dilution of this antibody dectected CDK5RAP2 in 10µg of HeLa cell lysate.
Target description
~220 kDa observed. Uniprot.org states four isoforms produced by alternative splicing, which may be observed at a molecular weight of ~216 kDa, ~211 kDa, ~210 kDa, and ~206 kDa in some cell lysates.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Alexis R Barr et al.
The Journal of cell biology, 189(1), 23-39 (2010-04-07)
The centrosomal protein, CDK5RAP2, is mutated in primary microcephaly, a neurodevelopmental disorder characterized by reduced brain size. The Drosophila melanogaster homologue of CDK5RAP2, centrosomin (Cnn), maintains the pericentriolar matrix (PCM) around centrioles during mitosis. In this study, we demonstrate a
Andrew Muroyama et al.
The Journal of cell biology, 213(6), 679-692 (2016-06-15)
Differentiation induces the formation of noncentrosomal microtubule arrays in diverse tissues. The formation of these arrays requires loss of microtubule-organizing activity (MTOC) at the centrosome, but the mechanisms regulating this transition remain largely unexplored. Here, we use the robust loss
Joo-Hee Sir et al.
The Journal of cell biology, 203(5), 747-756 (2013-12-04)
Most animal cells contain a centrosome, which comprises a pair of centrioles surrounded by an ordered pericentriolar matrix (PCM). Although the role of this organelle in organizing the mitotic spindle poles is well established, its precise contribution to cell division
Hitesh Patel et al.
Nature communications, 4, 2056-2056 (2013-06-28)
Kindlin-1 binds to integrins and regulates integrin activation at cell adhesions. Here we report a new function of Kindlin-1 in regulating spindle assembly. We show that Kindlin-1 localizes to centrosomes, its concentration peaking during G2/M, where it associates with various
Sadanori Watanabe et al.
The Journal of cell biology, 219(12) (2020-11-11)
Centrosomes, composed of centrioles that recruit a pericentriolar material (PCM) matrix assembled from PCNT and CDK5RAP2, catalyze mitotic spindle assembly. Here, we inhibit centriole formation and/or remove PCNT-CDK5RAP2 in RPE1 cells to address their relative contributions to spindle formation. While
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