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Key Documents

G002136

HPLC Analysis of Selegiline and Amphetamine Metabolites on Discovery® HS F5

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Discovery HS F5, 5 cm × 4.6 mm I.D., 5 μm particles (567513-U)
mobile phase: [A] water (10 mM ammonium acetate, pH 4.0 with acetic acid: [B] acetonitrile (v/v), (25:75, A:B)
flow rate: 1.0 mL/min
column temp.: 40 °C
detector: UV, 210 nm
injection: 10 μL
sample: 50 μg/mL each in mobile phase

suitability

application for HPLC

application(s)

clinical
forensics and toxicology

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

analytical column


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