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814475

TOYOPEARL® AF-Chelate-650M Bulk Media

phase IDA, iminodiacetic acid, bottle of 25 mL, 65 μm particle size

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About This Item

UNSPSC Code:
41115700

description

AF-Chelate-650M

product line

TOYOPEARL®

form

liquid

packaging

bottle of 25 mL

manufacturer/tradename

Tosoh TOYOPEARL® AF, 14475

extent of labeling

25-45 μeq/mL ligand density

parameter

3 bar max. pressure

technique(s)

HPLC: suitable

matrix

hydroxylated methacrylic polymer

matrix active group

IDA phase, iminodiacetic acid

particle size

65 μm

operating pH

2-12

separation technique

affinity

storage temp.

2-8°C

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General description

Toyopearl AF-Chelate-650 resin is derivatized with iminodiacetic acid for immobilized metal affinity chromatography or IMAC.IMAC works primarily by binding to histidine and free cysteine containing sequences of proteins or peptides.

Application

TOYOPEARL® Affinity Media are used in affinity chromatography, protein chromatography and activated/functionalized matrices.

Specifications

Clean in place with 0.5 M NaOH or 0.1 M HCl.

Physical form

Shipped in 20% (v/v) ethanol.

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Legal Information

Toyopearl is a registered trademark of Tosoh Corporation

related product

Product No.
Description
Pricing

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Hazard Classifications

Flam. Liq. 3

WGK

WGK 1

Flash Point(F)

103.1 °F

Flash Point(C)

39.5 °C

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S Yamauchi et al.
The Journal of biological chemistry, 274(4), 2456-2463 (1999-01-16)
Chondroitin 4-sulfotransferase, which transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate to position 4 of N-acetylgalactosamine in chondroitin, was purified 1900-fold to apparent homogeneity with 6.1% yield from the serum-free culture medium of rat chondrosarcoma cells by affinity chromatography on heparin-Sepharose CL-6B, Matrex
S Knör et al.
Journal of thrombosis and haemostasis : JTH, 6(3), 470-477 (2008-01-16)
Hemophilia A is currently treated by infusions of the coagulation factor (F) VIII, of which production and purification remain a challenging task. Current purification procedures using immunoaffinity chromatography are cumbersome, expensive, and suffer from the instability of the applied antibody
Zhenzhen Wen et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(20), 1732-1740 (2011-05-17)
In order to improve its stability, immobilized Concanavalin A (Con A) on Toyopearl adsorbents was conjugated with monomethoxy poly(ethylene glycol) succinimidyl propionate (mPEG-SPA) with different molecular weight. A colorimetric method using ninhydrin is proposed to determine the degree of PEGylation;

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