ZRB1927

Anti-Neutrophil Elastase Antibody, clone 1B2 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): EC:3.4.21.37;Bone marrow serine protease;Elastase-2;Human leukocyte elastase; HLE;Medullasin;PMN elastase
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1B2, recombinant monoclonal
• description: 1B2 Clone
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 28.52 kDa; observed mol wt ~32 kDa
• purified by: using Protein A
• species reactivity: human
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminus
• Protein ID accession no.: NP_001963
• UniProt accession no.: P08246
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... ELANE(1991)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1B2 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects human Neutrophil elastase. It targets an epitope within 14 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal region of human Neutrophil Elastase.

Application

Quality Control Testing

Evaluated by Western Blotting in U937 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Neutrophil Elastase in U937 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Neutrophil Elastase in lysates from THP-1 cells and Human bone marrow.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Neutrophil Elastase in human bone marrow tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound Neutrophil Elastase with a KD of 5.6 x 10-7 in an affinity binding assay.

Flow Cytometry Analysis: 0.1 μg from a representative lot detected Neutrophil Elastase in one million THP-1 cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Neutrophil Elastase in THP-1 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Neutrophil elastase (UniProt: P08246; also known as EC:3.4.21.37, Bone marrow serine protease, Elastase-2, Human leukocyte elastase, HLE, Medullasin, PMN elastase) is encoded by the ELANE (also known as ELA2) gene (Gene ID: 1991) in human. Neutrophils are the predominant circulating leukocyte population and are important in modulating innate and adaptive immunity. They serve as early responders and are recruited to sites of inflammation and infection by environmental cues. Neutrophil elastase (NE), a member of the peptidase S1 family, is synthesized with a signal peptide (aa 1-27) and a short propeptide (aa 28-29) that are subsequently cleaved off to produce the mature NE (aa 30-267). The mature form contains two glycans and four disulfide bonds. Release of NE can also stimulate adverse remodeling by degrading virtually all the components of the ECM in addition to elastin, and including collagen, fibronectin, and laminin. It is reported to cleave the triple-helix structure of type III and IV collagens, and cross-linked regions of type I, II, and V collagens, aggrecans, and fibronectin. NE-induced degradation of extracellular matrix (ECM) can release bioactive peptides and growth factors such as EGF and FGF that have mitogenic and motogenic effects on smooth muscle cells and fibroblasts. Increased activation of NE can lead to activation of matrix metalloproteinases that could potentiate ECM degradation. NE is also reported to cleave CXCL12, a chemokine involved in the regulated release of neutrophils from the bone marrow. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Taylor, S., et al. (2018). Front. Med. 5; 217; Sinha, S., et al. (1987). Proc. Natl. Acad. Sci. USA. 84(8); 2228-2232).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1