WH0025813M4

Monoclonal Anti-SAMM50 antibody produced in mouse

clone 2A9, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-CGI51, Anti-OMP85, Anti-SAM50, Anti-TOB55, Anti-TRG3, Anti-YNL026W, Anti-sorting and assembly machinery component 50 homolog (S. cerevisiae)
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 2A9, monoclonal
• form: buffered aqueous solution
• species reactivity: mouse
• technique(s): indirect ELISA: suitable; western blot: 1-5 μg/mL
• isotype: IgG2bκ
• GenBank accession no.: BC007830
• UniProt accession no.: Q8BGH2
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SAMM50(25813)

Description

General description

SAMM50 is a component of the sorting and assembly machinery (SAM) complex of the outer mitochondrial membrane. The SAM complex has a role in integrating beta-barrel proteins into the outer mitochondrial membrane (Humphries et al., 2005 [PubMed 15644312]).[supplied by OMIM

Immunogen

SAMM50 (AAH07830, 1 a.a. ~ 468 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Sequence
MGTVHARSLEPLPSSGPDFGGLGEEAEFVEVEPEAKQEILENKDVVVQHVHFDGLGRTKDDIIICEIGDVFKAKNLIEVMRKSHEAREKLLRLGIFRQVDVLIDTCQGDDALPNGLDVTFEVTELRRLTGSYNTMVGNNEGSMVLGLKLPNLLGRAEKVTFQFSYGTKETSYGLSFFKPRPGNFERNFSVNLYKVTGQFPWSSLRETDRGMSAEYSFPIWKTSHTVKWEGVWRELGCLSRTASFAVRKESGHSLKSSLSHAMVIDSRNSSILPRRGALLKVNQELAGYTGGDVSFIKEDFELQLNKQLIFDSVFSASFWGGMLVPIGDKPSSIADRFYLGGPTSVRGFSMHSIGPQSEGDYLGGEAYWAGLHLYTPLPFRPGQGGFGELFRTHFFLNAGNLCNLNYGEGPKAHIRKLAECIRWSYGAGIVLRLGNIARLELNYCVPMGVQTGDRICDGVQFGAGIRFL

Biochem/physiol Actions

Sorting and assembly machinery (SAM) 50 helps in aggregation of β-barrel proteins into the outer mitochondrial membrane (OMM). SAM 50, along with mitofilin and coiled-coil-helix-coiled-coil-helix domain containing 3 (CHCHD3), plays a vital role in maintaining mitochondrial shape, the morphology of cristae, and the assembly of respiratory complexes. Elevated expression of SAMM50 leads to mitochondrial fragmentation in HeLa cells under the influence of dynamin-related protein (Drp) 1. Depleted expression of SAM 50 affects assembly and steady-state level of voltage-dependent anion-selective channel (VDAC), which is essential for transport of metabolite across OMM.

Physical form

Solution in phosphate buffered saline, pH 7.4

Legal Information

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Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves