SBR00017

Puromycin aminonucleoside Ready Made Solution

10 mg/mL in water

• Synonym(s): 3′-Amino-3′-deoxy-N⁶,N⁶-dimethyladenosine
• Empirical Formula (Hill Notation): C₁₂H₁₈N₆O₃
• CAS Number: 58-60-6
• Molecular Weight: 294.31
• UNSPSC Code: 51281912
• NACRES: NA.85

Properties

• Assay: ≥98%
• Quality Level: 200
• form: liquid
• concentration: 10 mg/mL in water
• antibiotic activity spectrum: Gram-positive bacteria; neoplastics; parasites
• Mode of action: protein synthesis | interferes
• storage temp.: 2-8°C
• SMILES string: N[C@H]1[C@@H](O)[C@H](N(C=N2)C3=C2C(N(C)C)=NC=N3)O[C@@H]1CO
• InChI: 1S/C12H18N6O3/c1-17(2)10-8-11(15-4-14-10)18(5-16-8)12-9(20)7(13)6(3-19)21-12/h4-7,9,12,19-20H,3,13H2,1-2H3
• InChI key: RYSMHWILUNYBFW-UHFFFAOYSA-N

Description

Biochem/physiol Actions

Puromycin aminonucleoside is an aminonucleoside derivative of the known antibiotic puromycin. Puromycin aminonucleoside has been used in nephrology research, studying focal and segmental glomerulosclerosis, and in the induction of nephrosis in rats. Rats with puromycin aminonucleoside-induced nephrotic syndrome were used to study the excretion of sodium and NO_x metabolites.

Puromycin aminonucleoside has also been used to probe endothelial glycosaminoglycan synthesis in cultured glomerular endothelial cells and their relation to cell permeability. A puromycin aminonucleoside nephrosis model of rat glomerular disease was also used to study the role of the neuron-specific ubiquitin C-terminal hydrolase protein gene product 9.5 (PGP 9.5).

Preparation Note

Puromycin aminonucleoside solution is provided at 10 mg/mL in water and could be further diluted in aqueous buffers to a working concentration of 10 μg/mL (1:1000).

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable