SAB4200284

Anti-Neurocalcin-δ antibody produced in rabbit

~0.5 mg/mL, affinity isolated antibody

• Synonym(s): Anti-NCALD
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~22 kDa
• species reactivity: human, mouse, rat
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~0.5 mg/mL
• technique(s): western blot: 0.5-1.0 μg/mL using mouse brain extract (S1 fraction) and lysates of HEK-293T cells over-expressing human neurocalcin-δ.
• UniProt accession no.: P61601
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NCALD(83988) ; mouse ... Ncald(52589) ; rat ... Ncald(553106)

Description

General description

The neurocalcin-δ (NCALD) gene is mapped to human chromosome 8q22.3. The encoded protein belongs to a large family of neuronal calcium (Ca²⁺) sensor (NCS) proteins and is a member of visinin-like protein (VILIP) subfamily. Neurocalcin-δ is also one of the EF-hand-containing Ca²⁺-binding proteins. It is predominantly expressed in the Purkinje cells of the cerebellum and is also present in the olfactory epithelium.

Specificity

Anti-Neurocalcin-d specifically recognizes human and mouse neurocalcin-d.

Immunogen

synthetic peptide corresponding to a sequence located near the N-terminus of human neurocalcin-δ, conjugated to KLH. The corresponding sequence is identical in mouse and rat neurocalcin-δ.

Application

Anti-Neurocalcin-δ antibody produced in rabbit is suitable for immunoblotting.

Biochem/physiol Actions

Neurocalcin-δ (VILIP-3) is a neuronal calcium (Ca²⁺) sensor. It binds cooperatively to three Ca²⁺ ions. Neurocalcin-δ influences cyclic guanosine monophosphate (cGMP) signaling in the retina, by acting as a regulator of the retinal guanylate cyclase (retGC1)/ photoreceptor membrane guanylyl cyclase 1 (ROSGC1). Neurocalcin-δ interacts with retGC1 in a calcium (Ca2+) dependent manner. It colocalizes with retGC1 in neurons in the inner plexiform layer of the retina, in amacrine and ganglion cells where it may influence synaptic signaling processes. Neurocalcin-δ has been shown to interact with actin, clathrin, and α- and β-tubulins, indicating that it may be involved with clathrin-coated vesicle traffic. It interacts and activates the olfactory guanylate cyclase ONE-GC, suggesting that it may function in regulating the odorant signaling pathway.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 1% BSA as stabilizer and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable