SAB4200150
Anti-PIWIL3 (N-terminal) antibody produced in rabbit
~1.0 mg/mL
Synonym(s):
Anti-HIWI3, Anti-Piwi-like protein 3
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
~101 kDa
species reactivity
human
concentration
~1.0 mg/mL
technique(s)
immunohistochemistry: suitable using heat-retrieved formalin-fixed, paraffin-embedded human colon carcinoma sections and biotin/ExtrAvidin®-Peroxidase staining system
western blot: 1-2 μg/mL using lysates of HEK-293T cells over expressing human PIWIL3
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
General description
The PIWIL3 (Piwi like RNA-mediated gene silencing 3) protein belongs to the Piwi subfamily of Argonaute proteins. The Argonaute proteins contain a Piwi Argonaute-Zwille (PAZ) domain and PIWI domain. In humans, there are four PIWI proteins. The PIWIL3 gene is mapped to human chromosome 22q11.23. The Piwi proteins are mostly localized to the germline, where they bind to PIWI-interacting RNAs (piRNAs).
Specificity
Anti-PIWIL3 (N-terminal) recognizes human PIWIL3.
Application
Anti-PIWIL3 (N-terminal) antibody is also suitable for immunohistochemistry.
Anti-PIWIL3 (N-terminal) antibody produced in rabbit has been used in western blotting.
Biochem/physiol Actions
The P-element Induced WImpy testis (Piwi) proteins are important for the biogenesis and function of small non-coding RNAs. These proteins and PIWI-interacting RNAs (piRNAs) have been implicated in epigenetic control of gene expression, transposon silencing, gene expression, and translation regulation. The Piwi proteins significantly participate in germline development and gametogenesis of various metazoan species. These proteins undergo symmetrical dimethyl arginines (sDMAs) modification by protein methyltransferase 5 (PRMT5). The Piwi proteins are implicated in various cancers such as colorectal, breast, bladder, ovarian and non-small lung cancer and many more. Increased PIWIL3 gene expression has been observed in gastric cancer and metastatic melanoma.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Storage and Stability
Store at −20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation. Working dilutions should be discarded if not used within 12 hours.
Legal Information
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Wenhao Weng et al.
Biochimica et biophysica acta. Reviews on cancer, 1871(1), 160-169 (2019-01-02)
Piwi-interacting RNAs (piRNAs) are a very recently discovered class of small non-coding RNAs (ncRNAs), with approximately 20,000 piRNA genes already identified within the human genome. These short RNAs were originally described as key functional regulators for the germline maintenance and
Julia Höck et al.
Genome biology, 9(2), 210-210 (2008-02-29)
Argonaute proteins were first discovered genetically, and extensive research in the past few years has revealed that members of the Argonaute protein family are key players in gene-silencing pathways guided by small RNAs. Small RNAs such as short interfering RNAs
Travis Thomson et al.
Annual review of cell and developmental biology, 25, 355-376 (2008-01-10)
The evolutionarily conserved Argonaute/PIWI (AGO/PIWI, also known as PAZ-PIWI domain or PPD) family of proteins is crucial for the biogenesis and function of small noncoding RNAs (ncRNAs). This family can be divided into AGO and PIWI subfamilies. The AGO proteins
Yohei Kirino et al.
Nature cell biology, 11(5), 652-658 (2009-04-21)
Piwi family proteins are essential for germline development and bind piwi-interacting RNAs (piRNAs). The grandchildless gene aub of Drosophila melanogaster encodes the piRNA-binding protein Aubergine (Aub), which is essential for formation of primordial germ cells (PGCs). Here we report that
Charannya Sozheesvari Subhramanyam et al.
RNA biology, 17(11), 1613-1624 (2020-05-07)
PIWI homologs constitute a subclass of the Argonaute family. Traditionally, they have been shown to associate with a specific class of small RNAs, piRNAs, to suppress transposable elements and protect genomic integrity in germ cells. Recent studies imply that PIWI
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