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SAB1305538

Sigma-Aldrich

MONOCLONAL ANTI-HIS TAG antibody produced in mouse

clone 6AT18, IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Mouse Anti-Hexa Histidine tag

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

mouse

Quality Level

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

6AT18, monoclonal

form

buffered aqueous solution

technique(s)

western blot: 1:1000

isotype

IgG1

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Abgent′s anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.

Immunogen

Purified recombinant HIS-tagged fusion protein and poly-HIS peptide were used to produced this monoclonal antibody.

Physical form

Supplied in PBS with 0.09% (W/V) sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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