S6258
Spectinomycin ready made solution
100 mg/mL in DMSO/H2O, 1:1, Cell culture tested
Synonym(s):
Actinospectacin, Espectinomicina, Spectacin, Spectam, Togamycin, Trobicin
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352207
NACRES:
NA.76
Recommended Products
Related Categories
General description
Spectinomycin is an aminocyclitol antibiotic naturally produced by Streptomyces spectabilis. Spectinomycin shows in vitro inhibitory effect (31.2μg/mL) against gram-negative and gram-positive bacteria like Escherichia coli, Klebsiella, Enterobacter, and Staphylococcus epidermidis. It acts as a protein synthesis inhibitor by binding to the bacterium′s 30S ribosomal subunit. Specifically, it interacts with 16S rRNA during the elongation process and blocks elongation factor G (EF-G), which is responsible for the translocation catalysis of peptidyl-tRNA.
Spectinomycin is used as a selective resistance marker (Spc) in Leptospira spp. The Spc marker can be used for transformation screening in leptospires from both plasmids or chromosomal integration. Spectinomycin resistance occurs through mutation of rpsE gene which encodes the protein S5, or via the aadA bacterial gene which encodes the enzyme Aminoglycoside-3"-adenyltransferase. Spectinomycin can be also used in amplification of low copy number plasmid carrying replicons (100μg/mL).
In the clinic, Spectinomycin is used as a treatment against Neisseria gonorrhoeae for both gonorrheal urethritis and cervictis.
Spectinomycin is also a plastid protein synthesis inhibitor. It is used as a specific marker (0.2-5 mg/mL) for cell layer to monitor leaf development, and is also used to generate plants deficient for the plastid-encoded RNA polymerase on MS-agar media (0.5mg/mL).Spectinomycin was also studied for cattle respiratory tract infections by Pasteurella multocida and Mannheimia haemolytica.
Technical information
1. Spectinomycin ready made solution is formulated in order to prevent freezing at -20°C, and to avoid solution precipitation under freeze-thaw cycles.
2.The suggested concentration of Spectinomycin (based on literature) in cell culture assays is 0.2-100mγ/mL. Therefore, it is recommended to dilute the ready made solution 1:1000-500,000.
Spectinomycin is used as a selective resistance marker (Spc) in Leptospira spp. The Spc marker can be used for transformation screening in leptospires from both plasmids or chromosomal integration. Spectinomycin resistance occurs through mutation of rpsE gene which encodes the protein S5, or via the aadA bacterial gene which encodes the enzyme Aminoglycoside-3"-adenyltransferase. Spectinomycin can be also used in amplification of low copy number plasmid carrying replicons (100μg/mL).
In the clinic, Spectinomycin is used as a treatment against Neisseria gonorrhoeae for both gonorrheal urethritis and cervictis.
Spectinomycin is also a plastid protein synthesis inhibitor. It is used as a specific marker (0.2-5 mg/mL) for cell layer to monitor leaf development, and is also used to generate plants deficient for the plastid-encoded RNA polymerase on MS-agar media (0.5mg/mL).Spectinomycin was also studied for cattle respiratory tract infections by Pasteurella multocida and Mannheimia haemolytica.
Technical information
1. Spectinomycin ready made solution is formulated in order to prevent freezing at -20°C, and to avoid solution precipitation under freeze-thaw cycles.
2.The suggested concentration of Spectinomycin (based on literature) in cell culture assays is 0.2-100mγ/mL. Therefore, it is recommended to dilute the ready made solution 1:1000-500,000.
Physical form
Liquid - in 1:1 DMSO:water
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Alain Brans et al.
Applied and environmental microbiology, 70(12), 7241-7250 (2004-12-03)
The novel method described in this paper combines the use of blaI, which encodes a repressor involved in Bacillus licheniformis BlaP beta-lactamase regulation, an antibiotic resistance gene, and a B. subtilis strain (BS1541) that is conditionally auxotrophic for lysine. We
Spectinomycin hydrochloride (Trobicin) in the treatment of gonorrhoea. Observation of resistant strains of Neisseria gonorrhoeae.
A Reyn et al.
The British journal of venereal diseases, 49(1), 54-59 (1973-02-01)
Spectinomycin-resistant gonococcal infections in the United States, 1985-1986.
J M Zenilman et al.
The Journal of infectious diseases, 156(6), 1002-1004 (1987-12-01)
Hélène Bauby et al.
Microbiology (Reading, England), 149(Pt 3), 689-693 (2003-03-14)
In bacteria, the first reaction of the tryptophan biosynthetic pathway involves the conversion of chorismate and glutamine to anthranilate by the action of anthranilate synthase, which is composed of the alpha (trpE gene product) and beta (trpG gene product) subunits.
Dante Poggi et al.
Applied and environmental microbiology, 76(14), 4882-4885 (2010-06-01)
We measured the frequency of appearance of spontaneous mutants resistant to gentamicin, kanamycin, streptomycin, and spectinomycin in saprophytic and pathogenic Leptospira strains. The mutations responsible for the spontaneous resistance to streptomycin and spectinomycin were identified in the rpsL and rrs
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service