S1190

Anti-SLIPR/MAGI-3 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• MDL number: MFCD03792040
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 140-160 kDa
• species reactivity: rat
• technique(s): indirect immunofluorescence: 2.0 μg/mL using 293T cells transfected with recombinant Slipr.; microarray: suitable; western blot (chemiluminescent): 1.0 μg/mL using extracts of 293T cells transfected with recombinant Slipr
• UniProt accession no.: Q9JK71
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: rat ... Magi3(245903)

Description

General description

MAGI-3 possesses a guanylate-kinase-like region, six PDZ and two WW domains. This gene is located on human chromosome 1p13.

The MAGUK (Membrane Associated Guanylate kinase) family of proteins localize to regions of cell-cell contact, such as tight junctions in epithelial cells and synaptic junctions in neurons, and are believed to be involved in the assembly of multiprotein complexes via their protein-protein interaction domains.²

MAGI-3 (also termed SLIPR) was identified in a two hybrid screening as a protein interacting with the tumor suppressor PTEN,⁶ and Receptor Tyrosine Phosphatase β (RPTPβ).⁷

Specificity

Staining by immunoblotting and immunofluorescence is specifically inhibited by the immunizing peptide.

Immunogen

synthetic peptide corresponding to amino acids 835-850 of SLIPR (rat homolog of MAGI-3) conjugated to KLH via a N-terminal added lysine residue.

Biochem/physiol Actions

Membrane associated guanylate kinase, WW and PDZ domain containing 3 (MAGI3) serves as a scaffold protein for frizzled-4 and Ltap . It helps to control the c-Jun N-terminal kinase (JNK) signaling cascade.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.