R4001
Ribonuclease A-agarose
ammonium sulfate suspension, 400-1,000 units/g agarose (One ml gel will yield 12-30 units)
Synonym(s):
Ribonuclease I, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase
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biological source
protein from bovine pancreas
Quality Level
material
beaded agarose
form
ammonium sulfate suspension
specific activity
400-1,000 units/g agarose (One ml gel will yield 12-30 units)
mol wt
~13,700
storage temp.
2-8°C
Physical form
Suspension in 2.0 M (NH4)2SO4 pH 7.0
Analysis Note
Electrophoretically and enzymatically assayed. RNase A and B contents determined by SDS-PAGE. Protein determined by E1%/280.
inhibitor
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Description
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A spectrophotometric method for the measurement of ribonuclease activity.
M KUNITZ
The Journal of biological chemistry, 164(2), 563-568 (1946-08-01)
Pinaki P Misra et al.
Biopolymers, 97(12), 933-949 (2012-09-19)
In this study, we extensively report the effect of glycine betaine during the refolding of partially folded bovine α-lactalbumin (α-LA) in presence of hexadecyl trimethyl ammonium bromide (HTAB), and Ribonuclease A (RNAse A) in presence of sodium dodecyl sulfate (SDS)
Caterina Brandmayr et al.
Angewandte Chemie (International ed. in English), 51(44), 11162-11165 (2012-10-06)
Useful diversity: Quantification of modified tRNA nucleobases in different murine and porcine tissues reveals a tissue-specific overall modification content. The modification content correlates with rates of protein synthesis in vitro, suggesting a direct link between tRNA modification levels and tissue-specific translational
Shuangsheng Huang et al.
International journal of molecular medicine, 30(6), 1410-1416 (2012-10-03)
Tumor cells trigger angiogenesis through overexpression of various angiogenic factors including vascular endothelial growth factor (VEGF) and angiopoietin 1 (Ang1). Therefore, inhibition of the expression of both VEGF and Ang1, the initial step of tumor angiogenesis, is a promising strategy
Influence of thyroid state on cardiac and renal capillary density and glomerular morphology in rats.
Isabel Rodríguez-Gómez et al.
The Journal of endocrinology, 216(1), 43-51 (2012-10-11)
The purpose was to analyse the cardiac and renal capillary density and glomerular morphology resulting from a chronic excess or deficiency of thyroid hormones (THs) in rats. We performed histopathological, morphometrical and immunohistochemical analyses in hypothyroid and hyperthyroid rats to
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