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F3381

Sigma-Aldrich

Fast Red Violet LB Salt

Dye content ≥90%, Powder

Synonym(s):

5-Chloro-4-benzamido-2-methylbenzenediazonium chloride hemi(zinc chloride) salt

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About This Item

Linear Formula:
C14H11Cl2N3O · ½ZnCl2
CAS Number:
Molecular Weight:
376.31
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

Fast Red Violet LB Salt, Dye content ≥90 %

form

powder

composition

Dye content, ≥90%

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].Cc1cc(NC(=O)c2ccccc2)c(Cl)cc1[N+]#N

InChI

1S/C14H10ClN3O.ClH/c1-9-7-13(11(15)8-12(9)18-16)17-14(19)10-5-3-2-4-6-10;/h2-8H,1H3;1H

InChI key

SARKXLKWFKNUMR-UHFFFAOYSA-N

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General description

Fast Red Violet LB Salt, also known as 4′-amino-2′-chloro-5′-methylbenzanilide. It is a lipophilic amine with an aromatic system of moderate size and a hydrophilic diazonium cation. The diazonium salt is available commercially as a zinc chloride double salt. It comprises ≥90% dye content.

Application

  • Fast Red Violet LB Salt is suitable for quantitating alkaline phosphatase through histochemical, hematological, histological, and flow cytometric methods.
  • It has been used to study the role of TNF-α during fracture healing.
  • has been used for the detection of tartrate-resistant acid phosphatase (TRAP) in tissue sections.

Pictograms

Health hazardExclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Three-dimensional reconstruction of fracture callus morphogenesis.
Gerstenfeld LC
The Journal of Histochemistry and Cytochemistry, 54, 1215-1215 (2006)
Impaired fracture healing in the absence of TNF-alpha signaling: the role of TNF-alpha in endochondral cartilage resorption
Gerstenfeld, et al.
Journal of Bone and Mineral Research, 18(9), 1584?1592-1584?1592 (2003)
Conn's Biological Stains
Kiernan & Horobin
Conn?s Biological Stains (2002)
L C Gerstenfeld et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 18(9), 1584-1592 (2003-09-13)
TNF-alpha is a major inflammatory factor that is induced in response to injury, and it contributes to the normal regulatory processes of bone resorption. The role of TNF-alpha during fracture healing was examined in wild-type and TNF-alpha receptor (p55(-/-)/p75(-/-))-deficient mice.
Comparison of effects of the bisphosphonate alendronate versus the RANKL inhibitor denosumab on murine fracture healing.
Gerstenfeld LC
Journal of Bone and Mineral Research, 24, 196-196 (2009)

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