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BM21 human cell line

88052043, IHW Number: 9043

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About This Item

UNSPSC Code:
41106514

biological source

human blood

ethnicity

German/Italian

gender

male

growth mode

Suspension

karyotype

Not specified

morphology

Lymphoblastoid

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

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General description

Consanguineous: Yes

Cell Line Description

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

DNA Profile

A: 101
Cw: 1701
B: 4101
HLA DRA: 101
HLA DRB1: 1101
HLA DRB3: 202
HLA DQA1: 501
HLA DQB1: 301
HLA DPA1: 201
HLA DPB1: 1001

Serological Profile

HLA A: 1
HLA A ief: 1
HLA B: 41
HLA B ief: 41.1
Bw4/bw6: 6
HLA Dw: 5
HLA DR: 11
HLA Dw2: 25
HLA DR52: 52
HLA DQ: 7

Culture Medium

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Subculture Routine

Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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