OACM5.1 C cell line human

11012006, human esophagus (lung metastasis). Floating spheroids, some fibroblastoid morphology

• Synonym(s): Cell culture
• UNSPSC Code: 41106514

Properties

• product name: OACM5.1 C cell line human, 11012006
• biological source: human esophagus (lung metastasis)
• growth mode: Adherent
• karyotype: Hypodiploid 39-41 chromosomes
• morphology: Multicellular floating spheroids some with fibroblastoid morphology
• products: P53 mutation c.164delC c.743G>A
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): metastasis
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Barretts adenocarcinoma, adenocarcinoma of distal oesophagus

Application

Recommended culture medium: RPMI 1640 + 2mM L-Glutamine + 10% FBS

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10,13
D13S317: 11,12
D16S539: 10,11
D5S818: 11,12
D7S820: 8.2,8.2
THO1: 6,9.3
TPOX: 8
vWA: 19,20

Culture Medium

RPMI-1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 3 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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